Recombinant Mouse IL-25 (IL-17E) (ELISA Std.)

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Regulatory Status
Other Names
Interleukin 25, Interleukin 17E
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579809 4 pack £61
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This product is scheduled for discontinuation. Please use the availability checker to determine current availability. Cat. No. 587304 is available as an alternative.

IL-25 (also known as IL-17E) is a member of the IL-17 family, which includes IL-17A, IL-17B, IL-17C, IL-17F, and IL-17A/F. The primary sequence homology differs between family members, with mouse IL-17A and IL-17F having the highest homology (49%), while mouse IL-17A and IL-25 have the lowest (28%). IL-25 induces gene expression of IL-4, IL-5, and IL-13 in multiple tissues, and this induction results in T helper 2 (TH2)-type immune responses; this is characterized by an increase in serum IgE levels, pathologic changes in the lungs and digestive tract with eosinophilic infiltrates, an increase in mucus production, epithelial cell hyperplasia, and overall amplified allergic inflammation. IL-25 shares the receptor IL-17RB with IL-17B, although it binds with much higher affinity than IL-17B.

Product Details
Technical Data Sheet (pdf)

Product Details

Mouse IL-25, amino acids Val17-Ala169 (Accession# NM_080729) was expressed in insect cells
Molecular Mass
The 153 amino acid recombinant protein has a predicted molecular mass of 17.6 kD. This protein exists as a disulfide-linked homodimer. The DTT-reduced protein migrates at approximately 20 kD by SDS-PAGE. The non-reduced protein migrates as a homodimer, at approximately 40 kD by SDS-PAGE.
>95%, as determined by Coomassie stained SDS-PAGE
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
Immediately upon receipt, store unopened vials between 2°C and 8°C and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH 7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.

ELISA - Quality tested

Recommended Usage

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 1600 to 25 pg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This mouse IL-25 (IL-17E) protein is useful as a standard for a mouse IL-25 (IL-17E) sandwich ELISA, using unlabeled M3706F07 antibody (Cat. No. 676304) as capture and biotinylated M3704D11 antibody (Cat. No. 538104) as detection.

Antigen Details


IL-25 is produced by mast cells, epithelial cells, alveolar macrophages, microglia, and Th2 cells

IL-25 directly promotes Th2 cell differentiation and the expression of IL-4, IL-5, IL-13, CCL11 (eotaxin), and therefore, lung eosinophilia. IL-25 enhances IL-9 production by differentiated Th9 cells
CD4+ NKT cells, macrophages, endothelial cells, eosinophils, and Th2 and Th9 cells
IL-25 binds to IL-17RB
Antigen References
  1. Hurst SD, et al. 2002. J. Immunol. 169:443.
  2. Ikeda K, et al. 2003. Blood 101:3594.
  3. Kang CM, et al. 2005. Am. J. Respir. Cell. Mol. Biol. 33:290.
  4. Kleinschek ME, et al. 2007. J. Exp. Med. 204:161.
  5. Terashima A, et al. 2008. J. Exp. Med. 205:2727.
  6. Angkasekwinai P, et al. 2010. Nat. Immunol. 11:250.
Gene ID
140806 View all products for this Gene ID
View information about IL-25 on

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