Recombinant Mouse IL-25 (IL-17E) (ELISA Std.)

Pricing & Availability
Other Names
Interleukin 25, Interleukin 17E
Ave. Rating
Submit a Review
Product Citations
publications
Mouse_Il-25_ELISA_Std_RECOM_070119.png
  • Mouse_Il-25_ELISA_Std_RECOM_070119.png
Cat # Size Price Quantity Avail. Save
579809 4 pack £54
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

IL-25 (also known as IL-17E) is a member of the IL-17 family, which includes IL-17A, IL-17B, IL-17C, IL-17F, and IL-17A/F. The primary sequence homology differs between family members, with mouse IL-17A and IL-17F having the highest homology (49%), while mouse IL-17A and IL-25 have the lowest (28%). IL-25 induces gene expression of IL-4, IL-5, and IL-13 in multiple tissues, and this induction results in T helper 2 (TH2)-type immune responses; this is characterized by an increase in serum IgE levels, pathologic changes in the lungs and digestive tract with eosinophilic infiltrates, an increase in mucus production, epithelial cell hyperplasia, and overall amplified allergic inflammation. IL-25 shares the receptor IL-17RB with IL-17B, although it binds with much higher affinity than IL-17B.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Source
Mouse IL-25, amino acids Val17-Ala169 (Accession# NM_080729) was expressed in insect cells
Molecular Mass
The 153 amino acid recombinant protein has a predicted molecular mass of 17.6 kD. This protein exists as a disulfide-linked homodimer. The DTT-reduced protein migrates at approximately 20 kD by SDS-PAGE. The non-reduced protein migrates as a homodimer, at approximately 40 kD by SDS-PAGE.
Purity
>95%, as determined by Coomassie stained SDS-PAGE
Formulation
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Immediately upon receipt, store unopened vials between 2°C and 8°C and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH 7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Application

ELISA - Quality tested

Recommended Usage

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 1600 to 25 pg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This mouse IL-25 (IL-17E) protein is useful as a standard for a mouse IL-25 (IL-17E) sandwich ELISA, using unlabeled M3706F07 antibody (Cat. No. 676304) as capture and biotinylated M3704D11 antibody (Cat. No. 538104) as detection.

Antigen Details

Structure
Heterodimer
Distribution

IL-25 is produced by mast cells, epithelial cells, alveolar macrophages, microglia, and Th2 cells

Function
IL-25 directly promotes Th2 cell differentiation and the expression of IL-4, IL-5, IL-13, CCL11 (eotaxin), and therefore, lung eosinophilia. IL-25 enhances IL-9 production by differentiated Th9 cells
Interaction
CD4+ NKT cells, macrophages, endothelial cells, eosinophils, and Th2 and Th9 cells
Ligand/Receptor
IL-25 binds to IL-17RB
Antigen References
  1. Hurst SD, et al. 2002. J. Immunol. 169:443.
  2. Ikeda K, et al. 2003. Blood 101:3594.
  3. Kang CM, et al. 2005. Am. J. Respir. Cell. Mol. Biol. 33:290.
  4. Kleinschek ME, et al. 2007. J. Exp. Med. 204:161.
  5. Terashima A, et al. 2008. J. Exp. Med. 205:2727.
  6. Angkasekwinai P, et al. 2010. Nat. Immunol. 11:250.
Gene ID
140806 View all products for this Gene ID
UniProt
View information about IL-25 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/01/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

Login / Register
Forgot your password? Reset password?
Create an Account