Purified anti-LCAT Antibody

Pricing & Availability
Clone
Poly28648 (See other available formats)
Other Names
Phosphatidylcholine-sterol acyltransferase, Lecithin-cholesterol acyltransferase, Phospholipid-cholesterol acyltransferase
Isotype
Rabbit Polyclonal IgG
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Product Citations
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Poly28648_PURE_LCAT_Antibody_1_062919
Western blot of purified anti-LCAT antibody (Poly28648). Lane 1: Molecular weight marker; Lane 2: 20 µg of human liver lysate. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28648_PURE_LCAT_Antibody_1_062919
    Western blot of purified anti-LCAT antibody (Poly28648). Lane 1: Molecular weight marker; Lane 2: 20 µg of human liver lysate. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28648_PURE_LCAT_Antibody_2_062919
    IHC staining of purified anti-LCAT antibody (Poly28648) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
864801 25 µg £64
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Description

Human lecithin-cholesterol acyltransferase (LCAT) is a key enzyme in the metabolism of cholesterol in the plasma. It catalyzes the esterification of free cholesterol on circulating lipoproteins. In the brain, LCAT is mainly synthesized by astrocytes and it esterifies free cholesterol on nascent apolipoprotein E (apoE)-containing lipoproteins. The complete loss of LCAT in mice leads to a dramatic increase in apoE-HDL and reduced apolipoprotein in murine cerebrospinal fluid, suggesting its essential function in brain lipid metabolism.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant LCAT (Ile290-Pro432) with N-terminal His-Tag
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 - 2.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Human LCAT is a 440 amino acid protein with a molecular mass of 50 kD.
Distribution

Tissue distribution: Blood plasma, cerebral spinal fluid, brain, liver, and testes
Cellular distribution: Extracellular

Function
LCAT is involved in the metabolism of lipoproteins in the plasma and the brain.
Interaction
LCAT Interacts with APOA1.
Cell Type
Astrocytes
Biology Area
Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
Enzymes and Regulators
Antigen References
  1. Hirsch-Reinshagen V, et al. 2009. J Lipid Res. 50(5):885-93.
  2. Francone OL, et al. 1997. J Lipid Res.  38(4):813-22.
  3. Calabresi L, et al. 2010. Trends Cardiovasc Med.  20(2):50-3.
Gene ID
3931 View all products for this Gene ID
UniProt
View information about LCAT on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/28/2019

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