- Poly28648 (See other available formats)
- Other Names
- Phosphatidylcholine-sterol acyltransferase, Lecithin-cholesterol acyltransferase, Phospholipid-cholesterol acyltransferase
- Rabbit Polyclonal IgG
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- Product Citations
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Human lecithin-cholesterol acyltransferase (LCAT) is a key enzyme in the metabolism of cholesterol in the plasma. It catalyzes the esterification of free cholesterol on circulating lipoproteins. In the brain, LCAT is mainly synthesized by astrocytes and it esterifies free cholesterol on nascent apolipoprotein E (apoE)-containing lipoproteins. The complete loss of LCAT in mice leads to a dramatic increase in apoE-HDL and reduced apolipoprotein in murine cerebrospinal fluid, suggesting its essential function in brain lipid metabolism.Product Details
- Antibody Type
- Host Species
- Recombinant LCAT (Ile290-Pro432) with N-terminal His-Tag
- 0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
WB - Quality tested
IHC-P - Validated
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 - 2.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Human LCAT is a 440 amino acid protein with a molecular mass of 50 kD.
Tissue distribution: Blood plasma, cerebral spinal fluid, brain, liver, and testes
Cellular distribution: Extracellular
- LCAT is involved in the metabolism of lipoproteins in the plasma and the brain.
- LCAT Interacts with APOA1.
- Cell Type
- Biology Area
- Cell Biology, Neuroinflammation, Neuroscience
- Molecular Family
- Enzymes and Regulators
- Antigen References
- Hirsch-Reinshagen V, et al. 2009. J Lipid Res. 50(5):885-93.
- Francone OL, et al. 1997. J Lipid Res. 38(4):813-22.
- Calabresi L, et al. 2010. Trends Cardiovasc Med. 20(2):50-3.
- Gene ID
- 3931 View all products for this Gene ID
- View information about LCAT on UniProt.org
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