- Clone
- CPTAC-14d-58-11 (See other available formats)
- Other Names
- Ferritin Light Chain
- Isotype
- Rabbit IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Western blot of purified anti-Ferritin Light Chain antibody (clone CPTAC-14d-58-11). Lane 1: Molecular weight marker; Lane 2: 2.5 µg of human brain lysate; Lane 3: 5 µg of human brain lysate. The blot was incubated with 0.05 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP donkey anti-rabbit IgG antibody (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-Ferritin Light Chain antibody (clone CPTAC-14d-58-11) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm -
IHC staining of purified anti-Ferritin Light Chain antibody (clone CPTAC-14d-58-11) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 858901 | 25 µg | £75 | ||||
Ferritin is a ubiquitously expressed protein that controls iron storage and releases in a non-toxic form. The majority of ferritin in vivo is cytoplasmic, although iron-binding ferritin is also secreted into the serum. Clinically, plasma/serum ferritin has been used as a marker for total iron detection in the body, and thus could function as a diagnostic tool for iron-deficiency and anemia. Dysregulation of iron in the brain has been linked to Alzheimer’s disease. Recent studies have shown that ferritin light chain interacts with presenilin enhancer 2 (PEN-2) affecting its γ-secretase activity, which impacts amyloid beta (Aβ) production and deposition.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Rabbit
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.05 - 0.2 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.1 - 2.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2861086 (BioLegend Cat. No. 858901)
Antigen Details
- Structure
- Ferritin light chain is a 175 amino acid protein with a molecular mass of ~20 kD.
- Distribution
-
Tissue distribution: Ubiquitously expressed in all tissues
Cellular distribution: Cytoplasmic and extracellular - Function
- Ferritin light chain stores and buffers intracellular iron.
- Interaction
- Forms oligomers of 24 unit.
- Cell Type
- Astrocytes, Macrophages, Microglia, Oligodendrocytes
- Biology Area
- Angiogenesis, Neurodegeneration, Neuroinflammation, Neuroscience
- Molecular Family
- Neurotrophic Factors
- Antigen References
-
- Morris G, et al. 2018. Behav Brain Res. 341:154.
- Philpott CC, et al. 2017. J Biol Chem. 292(31):12764.
- Gene ID
- 2512 View all products for this Gene ID
- UniProt
- View information about Ferritin Light Chain on UniProt.org
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