Purified anti-Ferritin Light Chain Recombinant Antibody

Pricing & Availability
Clone
CPTAC-14d-58-11 (See other available formats)
Other Names
Ferritin Light Chain
Isotype
Rabbit IgG
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Product Citations
publications
CPTAC-14d-58-11_PURE_Ferr-Light-Chain_Antibody_1_031220
Western blot of purified anti-Ferritin Light Chain antibody (clone CPTAC-14d-58-11). Lane 1: Molecular weight marker; Lane 2: 2.5 µg of human brain lysate; Lane 3: 5 µg of human brain lysate. The blot was incubated with 0.05 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP donkey anti-rabbit IgG antibody (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • CPTAC-14d-58-11_PURE_Ferr-Light-Chain_Antibody_1_031220
    Western blot of purified anti-Ferritin Light Chain antibody (clone CPTAC-14d-58-11). Lane 1: Molecular weight marker; Lane 2: 2.5 µg of human brain lysate; Lane 3: 5 µg of human brain lysate. The blot was incubated with 0.05 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP donkey anti-rabbit IgG antibody (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • CPTAC-14d-58-11_PURE_Ferr-Light-Chain_Antibody_2_031220
    IHC staining of purified anti-Ferritin Light Chain antibody (clone CPTAC-14d-58-11) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • CPTAC-14d-58-11_PURE_Ferr-Light-Chain_Antibody_3_031220
    IHC staining of purified anti-Ferritin Light Chain antibody (clone CPTAC-14d-58-11) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # Size Price Quantity Avail. Save
858901 25 µg £75
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Description

Ferritin is a ubiquitously expressed protein that controls iron storage and releases in a non-toxic form. The majority of ferritin in vivo is cytoplasmic, although iron-binding ferritin is also secreted into the serum. Clinically, plasma/serum ferritin has been used as a marker for total iron detection in the body, and thus could function as a diagnostic tool for iron-deficiency and anemia. Dysregulation of iron in the brain has been linked to Alzheimer’s disease. Recent studies have shown that ferritin light chain interacts with presenilin enhancer 2 (PEN-2) affecting its γ-secretase activity, which impacts amyloid beta (Aβ) production and deposition.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Recombinant
Host Species
Rabbit
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.05 - 0.2 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.1 - 2.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2861086 (BioLegend Cat. No. 858901)

Antigen Details

Structure
Ferritin light chain is a 175 amino acid protein with a molecular mass of ~20 kD.
Distribution

Tissue distribution: Ubiquitously expressed in all tissues
Cellular distribution: Cytoplasmic and extracellular

Function
Ferritin light chain stores and buffers intracellular iron.
Interaction
Forms oligomers of 24 unit.
Cell Type
Astrocytes, Macrophages, Microglia, Oligodendrocytes
Biology Area
Angiogenesis, Neurodegeneration, Neuroinflammation, Neuroscience
Molecular Family
Neurotrophic Factors
Antigen References
  1. Morris G, et al. 2018. Behav Brain Res. 341:154.
  2. Philpott CC, et al. 2017. J Biol Chem. 292(31):12764.
Gene ID
2512 View all products for this Gene ID
UniProt
View information about Ferritin Light Chain on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03/12/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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