Purified anti-CAPNS1 Antibody

Pricing & Availability
Clone
Poly28643 (See other available formats)
Other Names
Calpain Small Subunit 1, Calcium-Activated Neutral Proteinase Small Subunit, CANP Small Subunit, Calpain, Small Polypeptide, CALPAIN4, CAPNS
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly28643_PURE_CAPNS1_Antibody_1_102219
Western blot of purified anti-CAPNS1 antibody (Poly28643). Lane 1: Molecular weight marker; Lane 2: 40 µg of human brain membrane lysate; Lane 3: 40 µg of mouse brain membrane lysate; Lane 4: 40 µg of rat brain membrane lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28643_PURE_CAPNS1_Antibody_1_102219
    Western blot of purified anti-CAPNS1 antibody (Poly28643). Lane 1: Molecular weight marker; Lane 2: 40 µg of human brain membrane lysate; Lane 3: 40 µg of mouse brain membrane lysate; Lane 4: 40 µg of rat brain membrane lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28643_PURE_CAPNS1_Antibody_2_102219
    IHC staining of purified anti-CAPNS1 antibody (Poly28643) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28643_PURE_CAPNS1_Antibody_3_102219
    IHC staining of purified anti-CAPNS1 antibody (Poly28643) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28643_PURE_CAPNS1_Antibody_4_102219
    IHC staining of purified anti-CAPNS1 antibody (Poly28643) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
864301 25 µg £64
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Description

Calpain Small Subunit 1 (CAPNS1) is a member of calpain small subunit family, which is a small regulatory subunit of calpains. Calpains are calcium-dependent cysteine-type proteinases that are involved in many cellular processes including cell proliferation, migration, adhesion and apoptosis. They are also associated with neurodegeneration, including myotonic dystrophy. Among its related pathways are apoptosis modulation and integrin pathway.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant Calpain Small Subunit 1 (CAPNS1) expressed in E.coli.
Formulation
0.01 M PBS, pH 7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.0 - 10 µg/mL in human and 5.0 - 10 µg/mL for mouse and rat is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
CAPNS1 is a 268 amino acid protein with an apparent molecular mass of ~28 kD.
Distribution

Tissue Distribution: Brain, kidney, liver, and immune system
Cellular Distribution: Cytosol, membrane, and nucleus

Function
CAPNS1 is involved in cell proliferation, adhesion, migration and apoptosis.
Interaction
Calpain catalytic subunit
Cell Type
B cells, Mesenchymal cells, Neurons, NK cells, T cells
Biology Area
Cancer Biomarkers, Cell Biology, Neurodegeneration, Neuroscience
Molecular Family
Enzymes and Regulators, Proteases
Antigen References
  1. Ma J, et al. 2012. J Biol Chem. 287(33):27480-9.
  2. Undyala VV, et al. 2008. J Cell Sci. 121:3581-8.
  3. Demarchi F, et al. 2006. J Cell Biol. 175(4):595-605.
Gene ID
826 View all products for this Gene ID 12336 View all products for this Gene ID 29156 View all products for this Gene ID
UniProt
View information about CAPNS1 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 10/22/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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