Purified anti-Adenosine Deaminase Antibody

Pricing & Availability
Clone
Poly28637 (See other available formats)
Other Names
Adenosine Deaminase, Adenosine Aminohydrolase, EC 3.5.4.4, ADA1
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly28637_Purified_Adenosine-Deaminase_Antibody_1_053119
Western blot of purified anti-Adenosine Deaminase antibody (Poly28637). Lane 1: Molecular weight marker; Lane 2: 5 µg of human Jurkat cell lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28637_Purified_Adenosine-Deaminase_Antibody_1_053119
    Western blot of purified anti-Adenosine Deaminase antibody (Poly28637). Lane 1: Molecular weight marker; Lane 2: 5 µg of human Jurkat cell lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28637_Purified_Adenosine-Deaminase_Antibody_2_053119
    IHC staining of purified anti-Adenosine Deaminase antibody (Poly28637) on formalin-fixed paraffin-embedded human spleen tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm.
Cat # Size Price Quantity Avail. Save
863701 25 µg £64
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Description

Adenosine Deaminase (ADA) is an enzyme that catalyzes hydrolytic deamination of adenosine and 2-deoxyadenosine. It is involved in purine metabolism, and plays an important role in adenosine homeostasis. ADA can also function as a positive regulator of T-cell coactivation and lymphocyte-epithelial cell adhesion. Mutations in ADA are linked to various human diseases, including autosomal recessive Omenn syndrome and congenital hemolytic anemia.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant Adenosine Deaminase expressed in E.coli.
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
ADA is a 363 amino acid protein with predicted molecular mass of ~41 kD and observed molecular mass of ~41 kD.
Distribution

Tissue Distribution: Spleen, T-cells, lymphocytes, and monocytes
Cellular Distribution: Cytosol

Function
ADA is involved in purine metabolism and adenosine homeostasis. It also functions as a positive regulator of T-cell coactivation and lymphocyte-epithelial cell adhesion.
Interaction
DPP4, ADORA1, ADORA2A
Cell Type
B cells, Lymphocytes, NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family
Enzymes and Regulators, Innate Immune Signaling
Antigen References
  1. Bradford KL, et al. 2017. J Clin Immunol. 37(7):626.
  2. Samuel CE. 2019. J Biol Chem. 294(5):1710.
Gene ID
100 View all products for this Gene ID 11486 View all products for this Gene ID 25367 View all products for this Gene ID
UniProt
View information about Adenosine Deaminase on UniProt.org

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Go To Top Version: 1    Revision Date: 05/31/2019

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