Loricrin Polyclonal Antibody, Purified

Pricing & Availability
Poly19051 (See other available formats)
Other Names
Covance Catalog# PRB-145P
Rabbit Polyclonal
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Product Citations
Immunohistochemistry staining with anti-loricrin (clone Poly19051) showing strong cytoplasmic staining of the granular layer of the epidermis in normal human skin tissue.
  • Purified_Loricrin_Antibody_IHC_021615
    Immunohistochemistry staining with anti-loricrin (clone Poly19051) showing strong cytoplasmic staining of the granular layer of the epidermis in normal human skin tissue.
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905101 100 µl £251
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Instructions for Use (pdf)

Product Details

Intended Use
In Vitro Diagnostic (IVD). Use in immunohistochemistry (IHC) test methods only.

The polyclonal antibody Poly19051 is used for the in vitro examination of human biological samples using immunohistochemistry (IHC) methods for the qualitative identification of Loricrin. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Human, Mouse
Antibody Type
Host Species
This monospecific polyclonal antibody was raised against a peptide sequence derived from the C-terminus of the mouse loricrin protein. It was subsequently purified using peptide affinity chromatography.
Phosphate-buffered solution+ 0.03% Thimerosal.
The antibody was purified by affinity chromatography.
1.0 mg/ml
Storage & Handling
When stored at ≤ -20°C, product is stable until the date shown on the label. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. If thawed and stored at 2-8°C, product is stable for 24 months from the date of thaw or until the expiry date on the label, whichever comes first.


Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
IHC: 1:1,000 with either biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).

Tissue Sections: Paraffin-embedded tissues
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Citrate Buffer Retrieval solution (Cat. No. 928502, 928602).
Incubation: 60 minutes at room temperature

Precautions for Use: Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.

Application Notes

This antibody is effective in immunohistochemistry (IHC). This antibody has been used successfully in both frozen and paraffin embedded tissues.

This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Wallace L, Roberts-Thompson L, and Reichelt J. Deletion of K1/K10 does not impair epidermal stratification but affects desmosomal structure and nuclear integrity. J. Cell Sci., 125: 1750-1758, Apr 2012. PubMed
  2. Bayo P, Sanchis A, Bravo A, Cascallana JL, Buder K, Tuckermann J, Schütz G, and PTrez P. Glucocorticoid Receptor Is Required for Skin Barrier Competence. Endocrinology, 149: 1377-1388, Mar 2008. [IHC] PubMed
  3. Hu Y, Baud V, Oga T, Kim KI, Yoshida K, Karin M. IKKalpha controls formation of the epidermis independently of NF-kappaB. Nature 410:710-714, 2001.
  4. Yuspa SH, Kilkenny AD, Steinert PM, Roop DR. Expression of murine epidermal differentiation markers is tightly regulated by restricted extracellular calcium concentrations in-vitro. J Cell Biol 109:1207-1217, 1989.
  5. Roop DR, Cheng CK, Titterington L, Meyers CA, Stanley JR, Steinert PM, Yuspa SH. Synthetic peptides corresponding to keratin subunits elicit highly specific antibodies. J Biol Chem 259:8037-8040, 1984. 6. Suzuki K, et al. 2009. Development. 136:367. (IHC) PubMed
  6. Croyle MJ, et al. Role of epidermal primary cilia in the homeostasis of skin and hair follicles. Development, 138: 1675-1685, May 2011. [IHC, IF] PubMed
  7. Sengupta A, et al. 2010. PLoS One. 5:12249. (IHC) PubMed
  8. Suzuki K, et al. 2009. Development. 136:367. (IHC) PubMed
  9. Liakath-Ali K, et al. 2014. Nat Commun. 11:3540. (IHC) PubMed
Product Citations
  1. Wallace L, et al. 2012. J Cell Sci. 125:1750-1758. PubMed
  2. Suzuki K, et al. 2009. Development. 136:367-372. PubMed
  3. Bayo P, et al. 2008. Endocrinology. 149:1377-1388. PubMed
  4. Liakath-Ali K, et al. 2014. Nat Commun. 5:3540. PubMed
  5. Croyle M, et al. 2011. Development. 138:1675-1685. PubMed
  6. Sengupta A, et al. 2010. PLoS One. 5:e12249. PubMed
  7. Wu N, et al. 2016. J Invest Dermatol. 136:192-201. PubMed
  8. Dahlhoff M, et al. 2016. Development. 143: 1823 - 1831. PubMed
  9. Matsui M, et al. 2016. J Pharmacol Sci. S1347-8613(16)30102-5. PubMed
  10. Naeem A, et al. 2016. J Allergy Clin Immunol. S0091-6749(16)31377-X. PubMed
  11. Shatirishvili M, et al. 2016. Cell Death Dis. 7:e2461. PubMed
  12. Carceller E, et al. 2016. Sci Rep. 6:38825. PubMed
  13. Madon-Simon M, et al. 2017. PLoS One. 12(6):e0180035. PubMed
  14. Zhang Y 2018. Cell stem cell. 23:516. PubMed
  15. Kousa YA, et al. 2017. Dev Dyn. 246:670. PubMed
  16. Suzuki S, et al. 2019. Life Sci Alliance. 2:. PubMed
  17. Devos M, et al. 2017. J Invest Dermatol. 137:494. PubMed
  18. Tripurani SK, et al. 2018. Mol Biol Cell. 29:2784. PubMed
  19. Lecland N, et al. 2019. Life Sci Alliance. 2:2. PubMed
Publication Library
AB_2565046 (BioLegend Cat. No. 905101)

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