HRP anti-BAF53b Antibody

Pricing & Availability
Clone
N332B/15 (See other available formats)
Other Names
Actin Like 6B, 53 KDa BRG1-Associated Factor B, Actin-Related Protein Baf53b, BRG1-Associated Factor 53B, ArpNalpha, ACTL6, Actin-Like Protein 6B, Actin-Like 6, HArpN Alpha
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
N332B-15_HRP_BAF53b_Antibody_1_083019_updated
Western blot of HRP anti-BAF53b antibody (N332B/15). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain nuclear fraction lysate; Lane 3: 20 µg of rat brain nuclear fraction lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
  • N332B-15_HRP_BAF53b_Antibody_1_083019_updated
    Western blot of HRP anti-BAF53b antibody (N332B/15). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain nuclear fraction lysate; Lane 3: 20 µg of rat brain nuclear fraction lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
  • N332B-15_HRP_BAF53b_Antibody_2_082019
    IHC staining of HRP anti-BAF53b antibody (N332B/15) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5.0 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • N332B-15_HRP_BAF53b_Antibody_3_082019
    IHC staining of HRP anti-BAF53b antibody (N332B/15) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5.0 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # Size Price Quantity Avail. Save
857403 25 µg £67
Check Availability


Need larger quantities of this item?
Request Bulk Quote
857404 100 µg £114
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

ACTL6/BAF53 proteins share similar homologies to actin-related proteins and are found within the SWI/SNF chromatin remodeling complexes. ACTL6b/BAF53b protein is part of the neural specific SWI/SNF complex, which play important roles in remodeling mononucleosomes in an ATP-dependent fashion, postmitotic neural development, dendritic growth, memory, and synaptic plasticity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Fusion protein corresponding to amino acids 39-114 of human BAF53b
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For IHC-P application, permeabilization with 0.25 % Triton X-100 is recommended.

RRID
AB_2814576 (BioLegend Cat. No. 857403)
AB_2814577 (BioLegend Cat. No. 857404)

Antigen Details

Structure
BAF53b is a 426 amino acid protein with a molecular mass of ~47 kD.
Distribution

Tissue Distribution: Moderately expressed in the brain and testis.
Cellular Distribution: Nucleus and cytosol.

Function
BAF53b is involved in the transcriptional activation / repression of select genes by remodeling mononucleosomes. BAF53b is also a component of the SWI/SNF chromatin remodeling complexes that drive enzymatic activites and chromatin structure alterations by changing the DNA-histone contacts within the nucleosome.
Interaction
BAF53 has been shown to interact with c-Myc.
Biology Area
Cell Biology, Neuroscience, Synaptic Biology, Transcription Factors
Antigen References
  1. Yoo M, et al. 2017. Journal of Neuroscience. 37(13):3686-3697.
  2. Vogel-Ciernia A, et al. 2013. Nat Neurosci. 16(5): 552–561.
  3. Zhan X, et al. 2015. Genesis. 53(7):440-8.
  4. Jeonghyeon Park, et al. 2002. Mol Cell Biol. 22(5): 1307–1316.
Gene ID
51412 View all products for this Gene ID
UniProt
View information about BAF53b on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/20/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account