HRP anti-α-Synuclein, aggregated Antibody

Pricing & Availability
Clone
A17183A (See other available formats)
Other Names
Non-A beta component of AD amyloid, NACP, PARK1, Synuclein alpha-140, non-A4 component of amyloid, alpha-synuclein, isoform NACP140, non-amyloid beta component (NAP)
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
A17183A_HRP_alpha-Synuclein_1_101519.png
IHC staining of HRP anti-α-Synuclein, aggregated antibody (clone A17183A) on formalin-fixed paraffin-embedded Parkinson’s disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • A17183A_HRP_alpha-Synuclein_1_101519.png
    IHC staining of HRP anti-α-Synuclein, aggregated antibody (clone A17183A) on formalin-fixed paraffin-embedded Parkinson’s disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • A17183A_HRP_alpha-Synuclein_2_101519.png
    IHC staining of HRP anti-α-Synuclein, aggregated antibody (clone A17183A) on formalin-fixed paraffin-embedded Parkinson’s disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • A17183A_HRP_alpha-Synuclein_3_101519.png
    IHC staining of HRP anti-α-Synuclein, aggregated antibody (clone A17183A) on formalin-fixed paraffin-embedded Parkinson’s disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2.5 µg/mL of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
864903 25 µg £71
Check Availability


Need larger quantities of this item?
Request Bulk Quote
864904 100 µg £158
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

α-synuclein is expressed principally in the nervous system, but it is also produced in the other tissues, including the skin. In the brain, the protein is primarily neuronal, but it is also present in glia. Neuronal α-synuclein is concentrated in the presynaptic nerve terminals, interacts with plasma membrane phospholipids, and is also present in the nuclei and mitochondria. At least three isoforms of synuclein are produced through alternative splicing. The most common isoform is a 140 amino acid-long transcript. Other isoforms are α-synuclein-126, lacking residues 41-54; and α-synuclein-112, which lacks residues 103-130. α-synuclein’s physiological role is poorly understood, but the protein has been implicated in regulating dopamine release and transport, synaptic vesicle clustering, and functioning as a SNARE-complex chaperone. α-Synuclein fibrils are a major component of the intracellular Lewy bodies that are associated with Parkinson's disease, Lewy body dementia, and multiple system atrophy.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human alpha-synuclein aggregates
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 2.5 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Human α-synuclein is a 140 amino acid protein with a molecular mass of 14 kD.
Distribution

Tissue distribution: Primarily nervous system, but lower expression in other tissues such as blood and skin.
Cellular distribution: Cytoskeleton, cytosol, lysosome, mitochondria, nucleus, plasma membrane, and extracellular.

Function
α-synuclein plays several roles in synaptic activity such as synaptic vesicle trafficking and neurotransmitter release. It is also involved in the regulation of gene expression.
Interaction
Interacts with various proteins, such as UCHL1, phospholipase D, histones and SNARE components.
Cell Type
Neurons
Biology Area
Cell Biology, Mitochondrial Function, Neurodegeneration, Neuroinflammation, Neuroscience, Protein Misfolding and Aggregation, Protein Trafficking and Clearance, Synaptic Biology
Molecular Family
α-Synuclein, Presynaptic proteins, Synaptic Vesicle Trafficking/Endocytosis
Antigen References
  1. Mor DE, et al. 2016. Neurobiol. Dis. 88:66.
  2. Jucker M and Walker LC. 2013. Nature. 501(7465):45.
  3. Bartels T, et al. 2011. Nature. 477(7362):107.
  4. Devine MJ, et al. 2011. Mov. Disord. 26:2160.
Gene ID
6622 View all products for this Gene ID
UniProt
View information about alpha-Synuclein on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/15/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account