HRP anti-α-Dystroglycan Antibody

Pricing & Availability
Clone
2238 (See other available formats)
Other Names
α-DG, αDG
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
2238_HRP_Alpha-Dystroglycan_Antibody_1_0622119.png
Western blot of HRP anti-α-Dystroglycan antibody (clone 2238). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of clone 2238 at 4°C. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • 2238_HRP_Alpha-Dystroglycan_Antibody_1_0622119.png
    Western blot of HRP anti-α-Dystroglycan antibody (clone 2238). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of clone 2238 at 4°C. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • 2238_HRP_Alpha-Dystroglycan_Antibody_2_0622119.png
    IHC staining of HRP anti-α-Dystroglycan antibody (clone 2238) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 2238_HRP_Alpha-Dystroglycan_Antibody_3_0622119.png
    IHC staining of HRP anti-α-Dystroglycan antibody (clone 2238) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 2238_HRP_Alpha-Dystroglycan_Antibody_4_0622119.png
    IHC staining of HRP anti-α-Dystroglycan antibody (clone 2238) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
852403 25 µg £74
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852404 100 µg £158
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Description

Dystrophin-associated glycoprotein 1 (DAG1) is a central component of dystrophin-glycoprotein complex that is encoded by the DAG1 gene. Dystroglycan links the cytoskeletal structure to the extracellular matrix, and thus plays an important role in several biological processes such as laminin and basement membrane assembly, sarcolemmal stability, nodal formation, migration and cell survival.

Dystroglycan is proteolytically cleaved to 2 functional peptides, α- and β-dystroglycan. Alpha-dystroglycan is the extracellular dystroglycan, which functions as a receptor for proteins containing laminin-G domains.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified bovine brain α-dystroglycan
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10.0 µg per ml. For formalin-fixed paraffin-embedded immunohistochemical staining, a concentration range of 5.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone works in WB on human, mouse and rat endogenous brain lysates.

Application References

(PubMed link indicates BioLegend citation)
  1. McDearmon EL, et al. 2006. FEBS Letters. 580(2006):3381 (WB)

Antigen Details

Structure
Dystroglycan is an 895 amino acid protein with a molecular mass of ~97 kD. α-dystroglycan, a cleavage product of dystroglycan, is a 624 amino acid protein with an observed molecular mass of ~104 kD.
Distribution

Tissue Distribution: High expression in brain, muscle and female tissues. Expressed in most tissues.
Cellular Distribution: Extracellular, Membrane

Function
Alpha-dystroglycan forms dystrophin-associated glycoprotein complex (DGX). It is nvolved in cell survival, lamini and basement membrane assembly and sarcolemmal stability, peripheral nerve myelination.
Interaction
Forms dystrophin-associated blycoprotein complex with beta-dystroglycan and dystrophin. Also interacts with sarcoglycans.
Ligand/Receptor
Functions as an extracellular receptor for ECM proteins that contains laminin-G domain.
Cell Type
Epithelial cells
Biology Area
Cell Adhesion, Cell Motility/Cytoskeleton/Structure, Neurodegeneration
Molecular Family
Adhesion Molecules, Scaffold Proteins
Antigen References
  1. McDearmon EL, et al. 2006. FEBS Letters. 580(2006):3381
  2. Endo T. 2015. J. Biochem. 157(1):1
Gene ID
1605 View all products for this Gene ID
UniProt
View information about alpha-Dystroglycan on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/21/2019

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