Direct-Blot™ HRP anti-STAT6 Phospho (Tyr641) Antibody

Pricing & Availability
Clone
A15137E (See other available formats)
Other Names
Signal transducer and activator of transcription 6, IL-4 Stat
Isotype
Mouse IgG1, κ
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publications
A15137E_DB-HRP_STAT6_Phospho_Antibody_041117
Total cell lysate (15 µg protein) from non-treated (lane 1) and 100 ng/mL of recombinant human IL-4 treated (lane 2) Daudi cells (10 minute treatment) were resolved by electrophoresis, transferred to nitrocellulose, and probed with 1:400 of purified anti-STAT6 Phospho antibody or 1:10000, 1:40000 and 1:100000 of Direct-Blot™ HRP anti-STAT6 Phospho antibody (upper) or loading control GAPDH (clone Poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG secondary antibody for the purified antibody, and HRP donkey anti-rabbit IgG antibody for GAPDH. Lane M is the molecular weight ladder.
  • A15137E_DB-HRP_STAT6_Phospho_Antibody_041117
    Total cell lysate (15 µg protein) from non-treated (lane 1) and 100 ng/mL of recombinant human IL-4 treated (lane 2) Daudi cells (10 minute treatment) were resolved by electrophoresis, transferred to nitrocellulose, and probed with 1:400 of purified anti-STAT6 Phospho antibody or 1:10000, 1:40000 and 1:100000 of Direct-Blot™ HRP anti-STAT6 Phospho antibody (upper) or loading control GAPDH (clone Poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG secondary antibody for the purified antibody, and HRP donkey anti-rabbit IgG antibody for GAPDH. Lane M is the molecular weight ladder.
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686015 100 µL £198
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Description

STAT6 is a member of the signal transducer and activator of transcription (STAT) family, activating gene expression in response to IL-4 and IL-13 stimulation. Upon cytokine stimulation, the receptor is phosphorylated by the associated Janus Kinases (Jak), followed by recruiting cytoplasmic STAT6. The Tyr641 residue of STAT6 is, in turn, phosphorylated by Jak. Phosphorylated STAT6 forms homodimers, translocates to the nucleus, and regulates transcription of target genes. STAT6 plays crucial roles in differentiation of T helper 2 (Th2) cells, class switch of immunoglobulins in B cells, expression of cell surface markers such as MHC class II, and the development of allergic inflammation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human STAT6 peptide phosphorylated at Tyr 641
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:10,000 - 1:100,000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

Human STAT6 has three isoforms; the molecular weights are 94, 82 and 74kD. The immunogen (phosphorylated peptide) is shared by these three isoforms. This antibody recognizes STAT6 Phospho (Tyr641) in all three isoforms. The predominant band detected is at 94 kD.

Clone A15137E does not react with mouse.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

RRID
AB_2687037 (BioLegend Cat. No. 686015)

Antigen Details

Structure
847 amino acids with a predicted molecular weight of 94 kD. Contains a SH2 domain responsible for phosphor-Tyrosine binding and dimerization.
Distribution

Cytoplasm; translocates to the nucleus when phosphorylated.

Function
STAT6 is a transcription factor, mediating IL-4 and IL-13 signaling. While STAT6 is widely expressed in human tissues, it exhibits elevated expression in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung, and placenta. Following phosphorylation by Janus kinases, STAT6 translocates to the nucleus where it regulates gene transcription.
Interaction
STAT6 interacts with CBP/p300 and NCOA1. Forms a homodimer or a heterodimer with other STAT family members.
Cell Type
B cells
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Phospho-Proteins, Nuclear Markers
Antigen References

1. Goenka S, et al. 2011. Immunol. Res. 50:87.
2. Wurster AL, et al. 2000. Oncogene 19:2577.
3. Akira S. 1999. Stem Cells 17:138.
4. Zamorano J, et al. 2005. J. Immunol. 174:2843.
5. David M, et al. 2001. Oncogene 20:6660.
6. Takeda K, et al. 1996. Nature 380:627.

Gene ID
6778 View all products for this Gene ID
UniProt
View information about STAT6 Phospho (Tyr641) on UniProt.org

Related FAQs

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Go To Top Version: 0    Revision Date: 04/19/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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