Product: APC anti-mouse Ly-6G |
Catalog No.: 127614 |
Jim Hsiao, PhD Washoington University in Saint Louis |
Great Tool to Detect Neutrophil in Lung | |||
Overall: | Product Quality: | Ease of Use: | |
Great tool for neutrophil detection. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | Single cells isolated from primary Balb/c lung graft |
Brief Protocol: | Cells were isolated and blocked with Fc block for 30 minutes. Cells were then stained with APC-Ly6G(1A8) antibody (1:120 in FACS buffer) for 30 minutes on ice. Cells were then briefly washed and analyzed by flow cytometry. |
Results Summary: | Was able to differentiate Ly6G+CD11c+ neutrophil in lung graft. |
Additional Notes: |
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