Product: Brilliant Violet 711™ anti-human CD14 |
Catalog No.: 301838 |
Anne Rissiek, Dr. Universitätsklinikum Hamburg-Eppendorf (UKE) |
Anti-human CD14 Brilliant Violet 711™ | |||
Overall: | Product Quality: | Ease of Use: | |
Good discrimination of monocytes in human whole blood. |
Experimental Design |
Application: | Flow Cytometry |
Cells used: | Human EDTA blood |
Brief Protocol: | 1. 100 µl EDTA blood plus 50 µl antibody cocktail (in FACS Buffer), incubate for 30 min at RT. 2. Lyse red good cells by adding 1 ml Lysis Buffer, incubate for 10 min at RT, wash 2x with PBS, 400 g, 5 min, trash supernatant. 3. Resuspend cells in 200-300 µl FACS Buffer, FACS analysis The CD14 antibody was titrated and applied at a final dilution of 1:300. |
Results Summary: | Good discrimination of monocytes in human whole blood. |
Additional Notes: | Gating strategy: Lymphocytes --> Single cells --> CD45+ --> CD14+. |
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