Product: Recombinant Human TNF-α (carrier-free)

Catalog No.: 570102

Maryan Rizk, Graduate Student UCSD

Worked Great! Used to induce NF-kB Activity in 293T cells (Recombinant Human TNF-α)
Overall:	Product Quality:	Ease of Use:
The protein worked great as a positive control for all my experiments to induce NF-kB activity, which was measured with a luciferase reporter gene as the readout.

Experimental Design

Application:	Induction of NF-kB as a positive control
Cells used:	HEK293T
Brief Protocol:	HEK293T cells were plated at 6x10^5 cells/well in a 6-well plate. The next day, the cells were transfected with NF-kB luciferase reporter plasmid and a house-keeping Renilla reporter plasmid as an internal control. 24-hrs post transfection, cells were treated with the TNF-a at 50 ng/ml for 2 hours. Cells were then harvested for lysis and detection of luciferase activity.
Results Summary:	Great fold inductions were observed when compared to the experimental samples and the negative control.
Additional Notes:	Works great for tissue culture and with no observable cytotoxicity.

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Publication Status This data has not been published.

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