Product: APC anti-human CD4 |
Catalog No.: 344613 |
Isabell Baumann, M.D. Immuology |
Very Good Attaining CD4+ Cells from Human Peripheral Blood | |||
Overall: | Product Quality: | Ease of Use: | |
This antibody provides a good separation of CD4+ cells in human peripheral blood. |
Experimental Design |
Application: | Flow Cytometry |
Cells used: | Human peripheral blood cells |
Brief Protocol: | 50ul of human peripheral blood was stained with the CD4 antibody. Cells were incubated for 30 minutes at room temperature. After incubation, cells were lysed using a lysing buffer. Then, samples were washed with 1ml PBS for three times and cells were resuspended in 300ul FACS buffer for direct usage for flow cytometry. |
Results Summary: | Very good separation of CD4+ and CD4- lymphocytes. |
Additional Notes: |
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