Product: Pacific Blue™ anti-human CD326 (EpCAM)

Catalog No.: 324218

Sarah Pierce, Undergraduate WIBR

EpCAM is Not Detected on SUM159 Cells
Overall:	Product Quality:	Ease of Use:
EpCAM is not expressed on the cell surface of SUM159 cells.

Experimental Design

Application:	Flow cytometry
Cells used:	SUM159
Brief Protocol:	Cells were trypsinized, neutralized with DMEM 10%, and re-suspended in ice-cold PBS with 2% IFS at 1x10^6 cells per 100 uL. The cells were incubated on ice with 1:100 antibody for 30’, washed with 9 times the original volume of ice-cold PBS- with 2% IFS, and re-suspended in 500 uL ice-cold PBS- with 2% IFS.
Results Summary:	EpCAM is not expressed by SUM159 cells.
Additional Notes:

Data Image Click on the image for full size data.

Publication Status This data has not been published.

Technical Service Notes

Trypsinization can affect epitopes since the process is enzyme-based. This can make it difficult for antibodies to find their target. This may explain the negative result. As such, we would suggest non-enzymatic or gentler methods to test and see if this result is consistent. Pacific Blue is also a very dim fluor, so a brighter one may be needed.

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