Product: CFSE Cell Division Tracker Kit
Catalog No.: 423801
Julia Dickow, M.Sc. Essen University Hospital

CFSE Cell Division Tracker Kit
Overall: Product Quality: Ease of Use:
Excellent cell tracer dye for CD8 T cell proliferation in vitro.
Experimental Design

Application: Proliferation Assay/FACS
Cells used: In vitro cultured CD8 T cells with DCs.
Brief Protocol: 1. Primary splenic CD8 T cells were isolated from mouse spleen. 2. CD8 T cells were diluted in RPMI medium (without FCS!!) to a concentration of 1 ∙ 107 cells per ml. 3. 1 µL of 5 mM CFSE stock solution per ml cells suspension was used. 4. Cells were incubated at 37 °C for 20 min. 5. Staining was stopped by adding 10 times the original staining volume of RPMI medium containing 10 % FCS.6. After centrifugation cells were used for proliferation assay in vitro.
Results Summary: Excellent cell tracer dye to follow cell divisions in cell culture in vitro.
Additional Notes:
Data Image

Click on the image for full size data.
Publication Status

This data has not been published.
See all Reviews
ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Forgot your password? Reset password?
Create an Account