Product: Brilliant Violet 510™ anti-human CD183 (CXCR3) |
Catalog No.: 353725 |
Felix Clanchy, Dr University of Oxford |
CD183 (CXCR3) Brilliant Violet 510™ | |||
Overall: | Product Quality: | Ease of Use: | |
CXCR3 expression is heterogeneously expressed on lymphocytes |
Experimental Design |
Application: | Flow Cytometry |
Cells used: | CD4+ cells isolated by MACS from human PBMC |
Brief Protocol: | Cells (200,000) were stained on ice in FACS buffer (1% FBS, 5mM EDTA, PBS) for 20min at a dilution of 1/400. The cells were then washed twice and analysed. In this assay the cells were stained with antibodies for CD4, CD45RO and CD183. |
Results Summary: | The antibody distinguishes CD183+ cells which, on CD4+ cells, were predominantly CD45RO+. |
Additional Notes: |
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