Tag-it Violet™ Proliferation and Cell Tracking Dye

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Other Names
Tag-It Violet Proliferation Cell Tracking Dye
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Tag-it_Violet_Cell_Proliferation_Cell_Tracking_Dye_1_FC_122115
Human peripheral blood mononuclear cells were stained with Tag-it Violet™ Proliferation and Cell Tracking dye, and then stimulated with PHA for four days. On day four, cells were harvested, stained with CD3-PE and the Tag-it Violet™ signal was analyzed by flow cytometry.
  • Tag-it_Violet_Cell_Proliferation_Cell_Tracking_Dye_1_FC_122115
    Human peripheral blood mononuclear cells were stained with Tag-it Violet™ Proliferation and Cell Tracking dye, and then stimulated with PHA for four days. On day four, cells were harvested, stained with CD3-PE and the Tag-it Violet™ signal was analyzed by flow cytometry.
  • Tag-it_Violet_Cell_Proliferation_Cell_Tracking_Dye_2_IF_122115
    Mouse spleen 72 hours after adoptive transfer of Tag-it Violet™-labeled splenocytes (purple). Nucleated cells are stained using 25 µM DRAQ™ (red). Image was captured at a 40X magnification.
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Cat # Size Price Quantity Avail. Save
425101 1 kit (5 vials, 122.25 µg per vial, 500 µl DMSO) 180€
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Description

Tag-it Violet™ Proliferation and Cell Tracking Dye can be used for cell tracking and for proliferation assays. Tag-it Violet™ passively diffuses into the cell where esterases cleave acetoxymethyl esters (AM) on the molecule, Tag-it Violet™ then covalently attaches to intracellular proteins enabling its long-term retention.

Tag-it Violet™ is excited with a fluorescence wavelength of 395 nm, and emits at 455 nm. Cells labeled wtih Tag-it Violet™ can be analyzed by flow cytometry in instruments equipped with a violet laser, or visualized by fluorescence microscopy using a DAPI filter.

Product Details
Technical data sheet

Product Details

Preparation
The Tag-it Violet™ Proliferation and Cell Tracking Dye is composed of lyophilized Tag-it Violet™ and anhydrous DMSO. For reconstitution, bring the kit to room temperature, add 50 µl of DMSO to one vial of Tag-it Violet™ dye until fully dissolved.
Storage & Handling
The Tag-it Violet™ Proliferation and Cell Tracking Dye should be stored at -20°C upon receipt. Do not open vials until needed. Once the DMSO is added to the Tag-it Violet™, use immediately, or store at -20°C in a dry place and protected ifrom light, preferably in a dessicator or in a container with dessicant for no more than one month. The unopened kit stored at -20°C is guaranteed for 1 year after purchase.
Application

FC - Quality tested
IF - Validated

Recommended Usage

This lot has been tested by flow cytometry for in vitro cell proliferation. It can be used at concentrations ranging from 1 - 20 µM for cell labeling. It is recommended that the reagent be titrated for optimal performance for each cell type, culturing condition, or application.

Application Notes

The molecular weight of Tag-it Violet™ Proliferation and Cell Tracking Dye is 489 kD. The maximum excitation and emission wavelengths of Tag-it Violet™ Proliferation and Cell Tracking Dye are 395 nm and 455 nm, respectively. Each 122.25 µg of Tag-it Violet™ Proliferation and Cell Tracking Dye may be reconstituted with 50 µl of anhydrous DMSO to yield a stock concentration of 5 mM.
 

Materials Provided:
5 vials x 122.25 µg Tag-it Violet™
500 µl anhydrous DMSO



Tag it-Violet™ Labeling Procedure:
1. Prior to reconstitution, spin down the vial of lyophilized reagent in a microcentrofuge to ensure the reagent is at the bottom of the vial.
2. Prepare stock solution by reconstituting 1 vial of lyophilized Tag-it Violet™ dye in 50 µl of anhydrous DMSO to make a 5 mM solution.
3. Prepare a 5 µM working solution by diluting 1 µL of 5 mM Tag-it Violet™ stock solution in 1 mL PBS for every 1 mL of cell suspension (or at an optimal working concentration as determined by titration).
4. Spin down and resuspend cells at 10-100 x 106 cells/mL in the Tag-it Violet™ working solution.
5. Incubate cells for 20 minutes at room temperature or at 37°C, and keep protected from light.
6. Quench the staining by adding five times the original staining volume of cell culture medium containing 10% FBS.
7. Pellet cells and resuspend in pre-warmed cell culture medium. 
8. Incubate cells for 10 minutes.
9. After incubation, Tag-it Violet™ labeled cells are ready for downstream applications or analysis.

Antigen Details

Structure
489 kD.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability, Neuroscience
Gene ID
NA

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Go To Top Version: 1    Revision Date: 12.21.2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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