Purified anti-mouse/human CD44 Antibody

Pricing & Availability
Clone
IM7 (See other available formats)
Other Names
Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Isotype
Rat IgG2b, κ
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Product Citations
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IM7_Pure_091907
C57BL/6 mouse splenocytes stained with purified IM7, followed by anti-rat IgG FITC
  • IM7_Pure_091907
    C57BL/6 mouse splenocytes stained with purified IM7, followed by anti-rat IgG FITC
  • IM7_pure_CD44_Antibody_2_IHC_102418
    Fresh, frozen mouse spleen was stained with purified CD44 clone IM7 conjugated and detected with a Cy3 CODEX™ oligonucleotide duplex (red). Samples were counterstained with CollagenIV Cy5 (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
Cat # Size Price Quantity Avail. Save
103001 50 µg 28€
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103002 500 µg 92€
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Description

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Squirrel Monkey, Horse (Equine), Cattle (Bovine, Cow), Swine (Pig, Porcine), Dog (Canine), Cat (Feline)
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Dexamethasone-induced myeloid leukemia M1 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, CyTOF® - Verified
ELISA, ICC, IHC-P, IP, CMCD, Stim - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD4417,18 that is located between amino acids 145 and 18620. This clone has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3, and in vivo inhibition of DTH4,5. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103046, 103065 - 103069).

Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.

Application References

(PubMed link indicates BioLegend citation)
  1. Trowbridge IS, et al. 1982. Immunogenetics 15:299. (ICFC, IP, CMCD)
  2. Katoh S, et al. 1994. J. Immunol. 153:3440. (ELISA)
  3. Budd RC, et al. 1987. J. Immunol. 138:3120. (IP)
  4. Camp RL, et al. 1993. J. Exp. Med. 178:497. (Block)
  5. Weiss JM, et al. 1997. J. Cell Biol. 137:1137. (Block)
  6. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC) PubMed
  7. Cuff CA, et al. 2001. J. Clin. Invest. 108:1031. (IHC)
  8. Lee JW, et al. 2006. Nature Immunol. 8:181.
  9. Zhang N, et al. 2005. J. Immunol. 174:6967. PubMed
  10. Huabiao C, et al. 2005. J. Immunol. 175:591. PubMed
  11. Gui J, et al. 2007. Int. Immunol. 19:1201. PubMed
  12. Wang XY, et al. 2008. Blood 111:2436. PubMed
  13. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  14. Yamazaki J, et al. 2009. Blood PubMed
  15. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  16. Chen YW, et al. 2010. Mol. Cancer Ther. 9:2879. PubMed
  17. Zheng Z, et al. 1995. J. Cell. Biol. 130:485.
  18. Wiranowska M, et al. 2010. Int. J. Cancer 127:532.
  19. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  20. Sandmaier BM, et al. 1998. Blood 91:3494.
  21. Yang Y, et al. 2015. Hypertension. 65:1047. PubMed
  22. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Zhang S, et al. 2017. Nature.. 10.1038/nature24283. PubMed
  2. Emmerson E, et al. 2018. EMBO Mol Med. 10.15252/emmm.201708051. PubMed
  3. Oyarce K, et al. 2018. Front Immunol. 9:112. PubMed
  4. An Y et al. 2018. Cell reports. 25(3):702-714 . PubMed
  5. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  6. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  7. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  8. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  9. Hinohara K, et al. 2018. Cancer Cell. 34:939. PubMed
  10. Zhou Z, et al. 2018. Cancer Res. 78:2248. PubMed
  11. Suzuki K, et al. 2019. Cell Mol Gastroenterol Hepatol. 8:119. PubMed
  12. Wang D, et al. 2019. Cancers (Basel). 11. PubMed
  13. Ding L, et al. 2019. Nat Commun. 10:4182. PubMed
  14. Emmerson E et al. 2017. eLife. 6 pii: e26620. PubMed
  15. Morabito KM, et al. 2017. Mucosal Immunol. 0.795138889. PubMed
  16. Kawabe T, et al. 2017. Sci Immunol. 2:eaam9315. PubMed
  17. Taylor NA, et al. 2017. J Clin Invest. 127:3472. PubMed
  18. Shirakawa K, et al. 2017. PLoS One. 12:e0186303. PubMed
  19. Cui X, et al. 2017. J Immunol. 199:4066. PubMed
  20. Reeves PM, et al. 2017. Oncotarget. 8:94040. PubMed
  21. Chartrand K, et al. 2018. Front Immunol. 1.642361111. PubMed
  22. Doorduijn EM, et al. 2018. Front Immunol. 0.416666667. PubMed
  23. Hoves S, et al. 2018. J Exp Med. 215:859. PubMed
  24. Yates K, et al. 2018. Proc Natl Acad Sci U S A. 115:2162. PubMed
  25. Sun H, et al. 2018. J Cell Biol. 217:1453. PubMed
  26. Pushalkar S, et al. 2018. Cancer Discov. 0.613194444. PubMed
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  28. Kaisar MMM, et al. 2018. PLoS Biol. 16:e2005504. PubMed
  29. Stelekati E, et al. 2018. Cell Rep. 2.445833333. PubMed
  30. Liu M, et al. 2018. Diabetologia. 61:2333. PubMed
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  36. Tan CL, et al. 2018. Immunohorizons. 0.248611111. PubMed
  37. Rosenbaum M, et al. 2019. Nat Commun. 2.05. PubMed
  38. Arnold IC, et al. 2019. PLoS Pathog. 15:e1007866. PubMed
  39. Noguerol J, et al. 2019. Biol Sex Differ. 10:47. PubMed
  40. Fiege JK, et al. 2019. PLoS Pathog. 15:e1008077. PubMed
  41. Abboud G, et al. 2018. Front Immunol. 9:1973. PubMed
  42. Carpenter SM, et al. 2017. PLoS Pathog. 13:e1006704. PubMed
  43. Englezou PC, et al. 2018. Mol Ther Nucleic Acids. 12:118. PubMed
  44. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  45. Kim SJ, et al. 2017. Nat Immunol. 18:1016. PubMed
  46. King IL, et al. 2017. Mucosal Immunol. 10:1160. PubMed
  47. Yoon BH, et al. 2018. Mol Cells. 41:953. PubMed
  48. Kakaradov B, et al. 2017. Nat Immunol. 18:422. PubMed
  49. Fujimoto N, et al. 2020. PLoS Biol. 18:e3000704. PubMed
  50. Tan L, et al. 2017. Int J Nanomedicine. 12:3095. PubMed
  51. Yi J, et al. 2019. Mol Cells. 42:228. PubMed
  52. Arkatkar T, et al. 2018. Kidney Int. 94:728. PubMed
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  55. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  56. Khairnar V, et al. 2018. Nat Commun. 2.153472222. PubMed
  57. Guo Y, et al. 2018. J Am Soc Nephrol. 29:2529. PubMed
  58. Chen H, et al. 2005. J Immunol. 175:591. PubMed
  59. He N 2005. J Immunol. 174:6967. PubMed
  60. Frank N, et al. 2005. Cancer Res. 65:4320. PubMed
  61. Gui J, et al. 2007. Int Immunol. 1.625694444. PubMed
  62. Kenna T, et al. 2008. Blood. 111:2091. PubMed
  63. Wang X, et al. 2008. Blood. 111:2436. PubMed
  64. Yamazaki J, et al. 2009. Blood. 114:2709. PubMed
  65. Walker F, et al. 2011. PLoS One. 6:e22733. PubMed
  66. Hirokawa Y, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 306:547. PubMed
  67. Yang Y, et al. 2015. Hypertension. 65:1047. PubMed
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RRID
AB_312952 (BioLegend Cat. No. 103001)
AB_312953 (BioLegend Cat. No. 103002)

Antigen Details

Structure
Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD
Distribution

All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells

Function
Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand/Receptor
Hyaluronan, MIP-1β, fibronectin, collagen
Cell Type
B cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al. 1995. Nat. Med. 1:558.
5. Hegde V, et al. 2008. J. Leukocyte Biol. 84:134.
6. Liu T, et al. 2009. Biol. Direct 4:40.

Gene ID
12505 View all products for this Gene ID 960 View all products for this Gene ID
UniProt
View information about CD44 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 10.24.2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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