Purified anti-human CD15 (SSEA-1) (Maxpar® Ready) Antibody

Pricing & Availability
Clone
W6D3 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
Lewis X, 3-FAL, 3-FL, LNFP III, Lex , SSEA-1, X-hapten
Isotype
Mouse IgG1, κ
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Product Citations
publications
W6D3_Purified_CD15_CyTOF_112213
Human PBMCs (blue) and RBC-lysed human whole blood cells (red) stained with 164Dy-anti-CD15 (W6D3). Data provided by DVS Sciences.
  • W6D3_Purified_CD15_CyTOF_112213
    Human PBMCs (blue) and RBC-lysed human whole blood cells (red) stained with 164Dy-anti-CD15 (W6D3). Data provided by DVS Sciences.
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323035 100 µg 97€
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Description

CD15 is 3-fucosyl-N-acetyllactosamine (3-FAL) also known as Lewis X, 3-FAL, X-hapten, and SSEA-1. CD15 is expressed on granulocytes and monocytes. It has also been shown to be expressed on Langerhans cells. CD15 has been implicated in adhesion as well as chemotaxis, phagocytosis, and bactericidal activity.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
WERI-RB-1 retinoblastoma cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified

Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

RRID
AB_2562831 (BioLegend Cat. No. 323035)

Antigen Details

Structure
Poly-N-acetyllactosamine
Distribution

Neutrophils, eosinophils, monocytes

Function
Adhesion
Cell Type
Embryonic Stem Cells, Eosinophils, Neutrophils
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Stocks SC, et al. 1990. Biochem. J. 268:275.

Gene ID
2526 View all products for this Gene ID
UniProt
View information about CD15 on UniProt.org

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD15 Reagents Request Custom Conjugation
Description Clone Applications
Brilliant Violet 785™ anti-human CD15 (SSEA-1) W6D3 FC
Purified anti-human CD15 (SSEA-1) W6D3 FC, CyTOF®
FITC anti-human CD15 (SSEA-1) W6D3 FC
PE anti-human CD15 (SSEA-1) W6D3 FC
APC anti-human CD15 (SSEA-1) W6D3 FC
Alexa Fluor® 488 anti-human CD15 (SSEA-1) W6D3 FC
Alexa Fluor® 647 anti-human CD15 (SSEA-1) W6D3 FC
PE/Cyanine5 anti-human CD15 (SSEA-1) W6D3 FC
PerCP anti-human CD15 (SSEA-1) W6D3 FC
PerCP/Cyanine5.5 anti-human CD15 (SSEA-1) W6D3 FC
Pacific Blue™ anti-human CD15 (SSEA-1) W6D3 FC
Alexa Fluor® 700 anti-human CD15 (SSEA-1) W6D3 FC
Brilliant Violet 510™ anti-human CD15 (SSEA-1) W6D3 FC
PE/Cyanine7 anti-human CD15 (SSEA-1) W6D3 FC
Brilliant Violet 605™ anti-human CD15 (SSEA-1) W6D3 FC
Brilliant Violet 650™ anti-human CD15 (SSEA-1) W6D3 FC
Purified anti-human CD15 (SSEA-1) (Maxpar® Ready) W6D3 FC, CyTOF®
PE/Dazzle™ 594 anti-human CD15 (SSEA-1) W6D3 FC
Brilliant Violet 421™ anti-human CD15 (SSEA-1) W6D3 FC
APC/Fire™ 750 anti-human CD15 (SSEA-1) W6D3 FC
FITC anti-human CD15 (SSEA-1) W6D3 FC
Pacific Blue™ anti-human CD15 (SSEA-1) W6D3 FC
TotalSeq™-A0392 anti-human CD15 (SSEA-1) W6D3 PG
Brilliant Violet 711™ anti-human CD15 (SSEA-1) W6D3 FC
APC/Cyanine7 anti-human CD15 (SSEA-1) W6D3 FC
TotalSeq™-B0392 anti-human CD15 (SSEA-1) W6D3 PG
TotalSeq™-C0392 anti-human CD15 (SSEA-1) W6D3 PG
PE anti-human CD15 (SSEA-1) W6D3 FC
APC/Fire™ 810 anti-human CD15 (SSEA-1) Antibody W6D3 FC
TotalSeq™-D0392 anti-human CD15 (SSEA-1) W6D3 PG
GMP FITC anti-human CD15 (SSEA-1) W6D3 FC
Spark YG™ 593 anti-human CD15 (SSEA-1) W6D3 FC
Go To Top Version: 3    Revision Date: 07.22.2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Brilliant Violet 785™ anti-human CD15 (SSEA-1)

  • Purified anti-human CD15 (SSEA-1)

  • FITC anti-human CD15 (SSEA-1)

  • PE anti-human CD15 (SSEA-1)

  • APC anti-human CD15 (SSEA-1)

  • Alexa Fluor® 488 anti-human CD15 (SSEA-1)

  • Alexa Fluor® 647 anti-human CD15 (SSEA-1)

  • PE/Cyanine5 anti-human CD15 (SSEA-1)

  • PerCP anti-human CD15 (SSEA-1)

  • PerCP/Cyanine5.5 anti-human CD15 (SSEA-1)

  • Pacific Blue™ anti-human CD15 (SSEA-1)

  • Alexa Fluor® 700 anti-human CD15 (SSEA-1)

  • Brilliant Violet 510™ anti-human CD15 (SSEA-1)

  • PE/Cyanine7 anti-human CD15 (SSEA-1)

  • Brilliant Violet 605™ anti-human CD15 (SSEA-1)

  • Brilliant Violet 650™ anti-human CD15 (SSEA-1)

  • Purified anti-human CD15 (SSEA-1) (Maxpar® Ready)

  • PE/Dazzle™ 594 anti-human CD15 (SSEA-1)

  • Brilliant Violet 421™ anti-human CD15 (SSEA-1)

  • APC/Fire™ 750 anti-human CD15 (SSEA-1)

  • FITC anti-human CD15 (SSEA-1)

  • Pacific Blue™ anti-human CD15 (SSEA-1)

  • TotalSeq™-A0392 anti-human CD15 (SSEA-1)

  • Brilliant Violet 711™ anti-human CD15 (SSEA-1)

  • APC/Cyanine7 anti-human CD15 (SSEA-1)

  • TotalSeq™-B0392 anti-human CD15 (SSEA-1)

  • TotalSeq™-C0392 anti-human CD15 (SSEA-1)

  • PE anti-human CD15 (SSEA-1)

  • APC/Fire™ 810 anti-human CD15 (SSEA-1) Antibody

  • TotalSeq™-D0392 anti-human CD15 (SSEA-1)

  • GMP FITC anti-human CD15 (SSEA-1)

  • Spark YG™ 593 anti-human CD15 (SSEA-1)

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