GMP Pacific Blue™ anti-human CD15 (SSEA-1) Antibody, CD15, W6D3

Pricing & Availability

Clone: W6D3 (See other available formats)
Workshop: HCDM listed
Other Names: Lewis X, 3-FAL, 3-FL, LNFP III, Lex , SSEA-1, X-hapten
Isotype: Mouse IgG1, κ
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Product Citations: publications

W6D3_GMP-Pacific-Blue_CD15_Antibody__112222 — Typical results from human peripheral blood granulocytes stained either with W6D3 Pacific Blue™ used at 5 µL/test (filled histogram) or with an isotype control (open histogram).

Compare all formats See GMP Pacific Blue™ spectral data

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260212

100 tests

287€

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Description

CD15 is 3-fucosyl-N-acetyllactosamine (3-FAL) also known as Lewis X, 3-FAL, X-hapten, and SSEA-1. CD15 is expressed on granulocytes and monocytes. It has also been shown to be expressed on Langerhans cells. CD15 has been implicated in adhesion as well as chemotaxis, phagocytosis, and bactericidal activity.

Product Details

Quality Statement This BioLegend’s GMP-RUO reagent is a fluorochrome-labeled monoclonal antibody single reagent for Research Use Only. Suitable for use in laboratory-developed multicolor flow cytometry tests. Single GMP-RUO products can be used in various multicolor flow cytometry combinations. Laboratory need to establish the GMP-RUO performance characteristics in combination with other reagents in normal and pathological samples.

Technical data sheet

Product Details

Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

WERI-RB-1 retinoblastoma cell line

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer.

Preparation

The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.

Concentration

200 µg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Violet Laser (405 nm)

RRID

AB_2928018 (BioLegend Cat. No. 260212)

Disclaimer

GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:

Batch-to-batch consistency
Material traceability
Documented procedures
Documented employee training
Equipment maintenance and monitoring records
Lot-specific certificates of analysis
Quality audits per ISO 13485:2016
QA review of released products

Antigen Details

Structure: Poly-N-acetyllactosamine
Distribution: Neutrophils, eosinophils, monocytes
Function: Adhesion
Cell Type: Embryonic Stem Cells, Eosinophils, Neutrophils
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Stocks SC, et al. 1990. Biochem. J. 268:275.
Gene ID: 2526 View all products for this Gene ID
UniProt: View information about CD15 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD15 Reagents Request Custom Conjugation

Description	Clone	Applications
Brilliant Violet 785™ anti-human CD15 (SSEA-1)	W6D3	FC
Purified anti-human CD15 (SSEA-1)	W6D3	FC,CyTOF®
FITC anti-human CD15 (SSEA-1)	W6D3	FC
PE anti-human CD15 (SSEA-1)	W6D3	FC
APC anti-human CD15 (SSEA-1)	W6D3	FC
Alexa Fluor® 488 anti-human CD15 (SSEA-1)	W6D3	FC
Alexa Fluor® 647 anti-human CD15 (SSEA-1)	W6D3	FC
PE/Cyanine5 anti-human CD15 (SSEA-1)	W6D3	FC
PerCP anti-human CD15 (SSEA-1)	W6D3	FC
PerCP/Cyanine5.5 anti-human CD15 (SSEA-1)	W6D3	FC
Pacific Blue™ anti-human CD15 (SSEA-1)	W6D3	FC
Alexa Fluor® 700 anti-human CD15 (SSEA-1)	W6D3	FC
Brilliant Violet 510™ anti-human CD15 (SSEA-1)	W6D3	FC
PE/Cyanine7 anti-human CD15 (SSEA-1)	W6D3	FC
Brilliant Violet 605™ anti-human CD15 (SSEA-1)	W6D3	FC
Brilliant Violet 650™ anti-human CD15 (SSEA-1)	W6D3	FC
Purified anti-human CD15 (SSEA-1) (Maxpar® Ready)	W6D3	FC,CyTOF®
PE/Dazzle™ 594 anti-human CD15 (SSEA-1)	W6D3	FC
Brilliant Violet 421™ anti-human CD15 (SSEA-1)	W6D3	FC
APC/Fire™ 750 anti-human CD15 (SSEA-1)	W6D3	FC
FITC anti-human CD15 (SSEA-1)	W6D3	FC
Pacific Blue™ anti-human CD15 (SSEA-1)	W6D3	FC
TotalSeq™-A0392 anti-human CD15 (SSEA-1)	W6D3	PG
Brilliant Violet 711™ anti-human CD15 (SSEA-1)	W6D3	FC
APC/Cyanine7 anti-human CD15 (SSEA-1)	W6D3	FC
TotalSeq™-B0392 anti-human CD15 (SSEA-1)	W6D3	PG
TotalSeq™-C0392 anti-human CD15 (SSEA-1)	W6D3	PG
PE anti-human CD15 (SSEA-1)	W6D3	FC
APC/Fire™ 810 anti-human CD15 (SSEA-1) Antibody	W6D3	FC
TotalSeq™-D0392 anti-human CD15 (SSEA-1)	W6D3	PG
GMP FITC anti-human CD15 (SSEA-1)	W6D3	FC
Spark YG™ 593 anti-human CD15 (SSEA-1)	W6D3	FC
GMP PE anti-human CD15 (SSEA-1)	W6D3	FC
GMP Pacific Blue™ anti-human CD15 (SSEA-1)	W6D3	FC
Spark Violet™ 500 anti-human CD15 (SSEA-1)	W6D3	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 12.12.2022

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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