Purified anti-human CD148 Antibody

Pricing & Availability
Clone
A3 (See other available formats)
Other Names
HPTP-eta, DEP-1, p260
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A3_Pure_021508
Human peripheral blood granulocytes stained with purified A3, followed by anti-mouse IgG FITC
  • A3_Pure_021508
    Human peripheral blood granulocytes stained with purified A3, followed by anti-mouse IgG FITC
Cat # Size Price Quantity Avail. Save
328702 100 µg 80€
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Description

CD148, a receptor-like protein tyrosine phosphatase also known as human protein tyrosine phosphatase-eta (HPTP-eta) or density-enhanced protein tyrosine phosphatase-1 (DEP-1), is involved in signal transduction in leucocytes and is thought to contribute to mechanisms of cellular differentiation. In lymphoid organs, CD148 was found to be widely expressed on B and T cells, granulocytes, macrophages, certain dendritic cells as well as mature thymocytes. The cellular level of CD148 was increased after in vitro activation of peripheral blood leucocytes. CD148 as a leucocyte activation marker and may be involved in the regulation of T cell activation. Leucocytes expressing CD148 are significantly upregulated in inflamed tissues and that a subset of these cells co-expresses the activation marker CD25. In non-lymphoid tissues, CD148 was found to be present on many epithelial cell types with glandular and/or endocrine differentiation as well as on fibrocytes, melanocytes and Schwann cells. Among non-hematopoietic cells, CD148 is expressed by characteristic types of epithelial and non-epithelial cells. Downregulation of CD148 might promote dedifferentiation and autonomous growth of such cells in malignant tumors.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
PHA-stimulated human PBMC
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IF - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Tangye SG. 1998. J. Immunol. 161:3249. (FC)
  2. Tangye SG. 1998. J. Immunol. 161:3803.
  3. Tangye SG. 1998. J. Exp. Med. 188:1691.
  4. Autschbach F. 1999. Tissue Antigens 54:485.
  5. Lin J and Weiss A. 2003. J. Cell Biol. 162:673. (IF)
  6. Whiteford, JR., et al. 2011. Mol Cell Biol. 22:3609. PubMed.
Product Citations
  1. Whiteford J, et al. 2011. Mol Biol Cell. 3.422916667. PubMed
Publication Library

Antigen Details

Distribution

Monocytes, T cells, B cells, NK cells, epithelial cells, fibrocytes, melanocytes and Schwann cells

Cell Type
B cells, Epithelial cells, Fibroblasts, Monocytes, NK cells, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
5796 View all products for this Gene ID
UniProt
View information about CD148 on UniProt.org

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Go To Top Version: 2    Revision Date: 02.04.2015

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