- A3 (See other available formats)
- Other Names
- HPTP-eta, DEP-1, p260
- Mouse IgG1, κ
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- Product Citations
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CD148, a receptor-like protein tyrosine phosphatase also known as human protein tyrosine phosphatase-eta (HPTP-eta) or density-enhanced protein tyrosine phosphatase-1 (DEP-1), is involved in signal transduction in leucocytes and is thought to contribute to mechanisms of cellular differentiation. In lymphoid organs, CD148 was found to be widely expressed on B and T cells, granulocytes, macrophages, certain dendritic cells as well as mature thymocytes. The cellular level of CD148 was increased after in vitro activation of peripheral blood leucocytes. CD148 as a leucocyte activation marker and may be involved in the regulation of T cell activation. Leucocytes expressing CD148 are significantly upregulated in inflamed tissues and that a subset of these cells co-expresses the activation marker CD25. In non-lymphoid tissues, CD148 was found to be present on many epithelial cell types with glandular and/or endocrine differentiation as well as on fibrocytes, melanocytes and Schwann cells. Among non-hematopoietic cells, CD148 is expressed by characteristic types of epithelial and non-epithelial cells. Downregulation of CD148 might promote dedifferentiation and autonomous growth of such cells in malignant tumors.Product Details
- Antibody Type
- Host Species
- PHA-stimulated human PBMC
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
(PubMed link indicates BioLegend citation)
- Tangye SG. 1998. J. Immunol. 161:3249. (FC)
- Tangye SG. 1998. J. Immunol. 161:3803.
- Tangye SG. 1998. J. Exp. Med. 188:1691.
- Autschbach F. 1999. Tissue Antigens 54:485.
- Lin J and Weiss A. 2003. J. Cell Biol. 162:673. (IF)
- Whiteford, JR., et al. 2011. Mol Cell Biol. 22:3609. PubMed.
AB_2174826 (BioLegend Cat. No. 328708)
Monocytes, T cells, B cells, NK cells, epithelial cells, fibrocytes, melanocytes and Schwann cells
- Cell Type
- Monocytes, T cells, B cells, NK cells, Epithelial cells, Fibroblasts, Tregs
- Biology Area
- Molecular Family
- CD Molecules
- Gene ID
- 5796 View all products for this Gene ID
- View information about CD148 on UniProt.org
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
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