PE anti-human CD179b (Ig λ5) Antibody

Pricing & Availability
Clone
HSL11 (See other available formats)
Other Names
Ig Lambda 5, λ5, Ig omega
Isotype
Mouse IgG1, κ
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Product Citations
publications
HSL11_PE_081210
Human cell lines REH (pro-B, blue), NALM-6 (pre-B, orange) and Ramos (B cell, open) stained with HSL11 PE
  • HSL11_PE_081210
    Human cell lines REH (pro-B, blue), NALM-6 (pre-B, orange) and Ramos (B cell, open) stained with HSL11 PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
349803 25 tests 96€
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349804 100 tests 208€
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Description

CD179b, also known as Ig Lambda 5, λ5, and Ig omega, is a 22 kD, C-like protein member within the Ig superfamily. λ5 associates with CD179a (VpreB) to form a surrogate light (SL) chain that in turn associates with the IgM heavy chain and the CD79a/CD79b heterodimer to form the pre-B cell receptor (PreBCR) complex. The function of CD179a/CD179b is still under investigation, though it has been reported that the deficiency of CD179a and/or CD179b impairs the maturation of the B cell precursors beyond the pre-B cell stage.

Product Details
Technical data sheet

Product Details

Reactivity
Human, African Green
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1 and immunohistochemistry of frozen and paraffin-embedded samples2.

Application References

(PubMed link indicates BioLegend citation)
  1. Tsyganezawa K, et al. 1998. Blood 92:4317. (IP)
  2. Kiyokawa N, et al. 2004. Modern Pathol. 17:423. (ICFC, IHC)
RRID
AB_10643420 (BioLegend Cat. No. 349803)
AB_10643421 (BioLegend Cat. No. 349804)

Antigen Details

Structure
Immunoglobine (Ig) V-like protein. Molecular weight 22 kD. Associates with CD179a (VpreB) to form the surrogate light (SL) chain, which binds to the the Ig µ heavy chain and CD79a/CD79b heterodimer complex to form the pre-B cell receptor (preBCR).
Distribution

Pro-B and early Pre-B cells

Cell Type
B cells
Biology Area
Cell Biology, Immunology, Signal Transduction, Costimulatory Molecules
Molecular Family
CD Molecules
Antigen References

1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers Wiley-Liss A John Wiley & Sons Inc.
2. Zhang M, et al. 2004. Cell. Mol. Immunol. 1:89.
3. Hendriks RW and Middendorp S. 2004. Trends Immunol. 25:249.

Gene ID
3543 View all products for this Gene ID
UniProt
View information about CD179b on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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