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Brilliant Violet 421™ anti-mouse IgM Antibody

Pricing & Availability
Clone
RMM-1 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin M
Isotype
Rat IgG2a, κ
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Product Citations
publications
RMM-1_BV421_1_051911
C57BL/6 splenocytes were stained with CD19 FITC and anti-IgM (clone RMM-1) Brilliant Violet 421™ (top) or rat IgG2a Brilliant Violet 421™ isotype control (bottom).
  • RMM-1_BV421_1_051911
    C57BL/6 splenocytes were stained with CD19 FITC and anti-IgM (clone RMM-1) Brilliant Violet 421™ (top) or rat IgG2a Brilliant Violet 421™ isotype control (bottom).
  • RMM-1_BV421_2_051911
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
406517 125 µL 141€
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406532 50 µg 194€
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406518 500 µL 304€
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Description

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µg size: 0.2 mg/mL
µL sizes: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. Some formats of the RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.

Application References

(PubMed link indicates BioLegend citation)
  1. Tertilt C, et al. 2009. Infect. Immun. 77:3044. (ELISA) PubMed
Product Citations
  1. Pani F, et al. 2021. Endocrinology. 162:. PubMed
  2. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  3. Shikatani EA et al. 2019. Cell Rep. 27(8):2304-2312 . PubMed
  4. Liang Q, et al. 2022. iScience. 25:104043. PubMed
  5. Geng T, et al. 2022. Methods Mol Biol. 2585:71. PubMed
  6. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  7. Zhu Y, et al. 2022. Clin Transl Med. 12:e887. PubMed
  8. Knizkova D, et al. 2022. Nat Immunol. 23:1644. PubMed
  9. Weber G, et al. 2014. J Exp Med. 211:1243. PubMed
  10. Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed
  11. Ghosh D, et al. 2016. J Immunol. 197: 1788 - 1800. PubMed
  12. Yamada H, et al. 2018. J Immunol. 201:3492. PubMed
  13. Wilmore JR, et al. 2022. Front Immunol. 12:791095. PubMed
  14. Hong JP, et al. 2020. Cell Reports Medicine. 1(3):100035. PubMed
  15. Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed
  16. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
  17. Spanier J, et al. 2016. Nat Commun. 7:11804. PubMed
RRID
AB_10899576 (BioLegend Cat. No. 406517)
AB_2650930 (BioLegend Cat. No. 406532)
AB_2561444 (BioLegend Cat. No. 406518)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type
B cells
Biology Area
Immunology
Gene ID
16019 View all products for this Gene ID
UniProt
View information about IgM on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 2    Revision Date: 01.29.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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