- 12G5 (See other available formats)
- Regulatory Status
- VII 70204
- Other Names
- CXCR4, Fusin
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD184, also known as fusin or CXCR4, is a 45 kD seven transmembrane G-protein-linked CXC chemokine receptor. CD184 is widely expressed on blood and tissue cells, including B and T cells, monocytes, macrophages, dendritic cells, granulocytes, megakaryocytes/platelets, lymphoid, myeloid precursor cells, endothelial cells, epithelial cells, astrocytes, and neurons, among other tissue cells. CD184 is the receptor for CXC chemokine SDF-1, mediates blood cell migration, and is involved in B lymphopoiesis and myelopoiesis, cardiogenesis, blood vessel formation, and cerebellar development. CXCR4 is also a coreceptor of X4 HIV-1 and an alternative receptor for some isolates of HIV-2.Product Details
- Human, African Green, Baboon, Chimpanzee, Cynomolgus, Rhesus, Sooty Mangabey
- Antibody Type
- Host Species
- CP-MAC-infected Sup-T1 cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
- Excitation Laser
Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections11, immunocytochemistry3, immunofluorescence microscopy2,6, and blocking of CD4-independent infection by HIV-2 and CD4-dependent infection by some T cell-tropic isolates of HIV-14,5. Clone 12G5 may not be suitable for Western blotting.10 The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 306539 & 306540).
(PubMed link indicates BioLegend citation)
- McKnight A, et al. 1997. J. Virol. 71:1692.
- Endres MJ, et al. 1996. Cell 87:745. (Immunogen, IF)
- Volin MV, et al. 1998. Biochem. Biophys. Res. Commun. 242:46. (ICC)
- Berndt C, et al. 1998. P. Natl. Acad. Sci. USA 95:12556. (Block)
- Ullrich CK, et al. 2000. Blood 96:1438. (Block)
- Murga M, et al. 2005. Blood 105:1992. (IF)
- Thompson BD. 2007. J. Biol. Chem. 282:9547. (FC) PubMed
- Isnardi I, et al. 2010. Blood 115:5026. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Fischer T, et al. 2008. PLoS One 3:e4069.
- Schmid BC, et al. 2004. Breast Cancer Res. Treat. 84:247. (IHC)
- Product Citations
AB_10901163 (BioLegend Cat. No. 306517)
AB_11146018 (BioLegend Cat. No. 306518)
- Rhodopsin family, G-protein linked seven transmembrane glycoprotein, 45 kD
T cells and B cells, dendritic cells, monocytes, granulocytes, hematopoietic progenitors, endothelial cells
- B lymphopoiesis and myelopoiesis, cardiogenesis, blood vessel formation, cerebellar development
- SDF-1 receptor, coreceptor for X4 HIV-1
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Granulocytes, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Monocytes, Neural Stem Cells, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
1. Berger E, et al. 1999. Annu. Rev. Immunol. 17:657.
2. Loetscher P, et al. 2000. Adv. Immunol. 74:127.
3. Murphy P, et al. 2000. Pharmacol. Rev. 52:145.
- Gene ID
- 7852 View all products for this Gene ID
- View information about CD184 on UniProt.org
- What is the F/P ratio range of our BV421™ format antibody reagents?
It is lot-specific. On average it ranges between 2-4.
- Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.
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