Biotin anti-human Granulysin Antibody

Pricing & Availability
DH10 (See other available formats)
Regulatory Status
Other Names
GNLY, 519, LAG2, NKG5, LAG-2, D2S69E, TLA519
Mouse IgG1, κ
Ave. Rating
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Product Citations
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526103 50 µg 141€
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526104 500 µg 465€
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Granulysin is a cytolytic protein present in granules of human natural killer (NK) cells and activated cytotoxic T lymphocytes (CTLs). Granulysin is synthesized as a 15 kD precursor, which is then cleaved at both the amino- and carboxy-termini into a 9 kD activated form.

The 9 kD granulysin is released into the intercellular space between target and effector cells via a granule exocytosis pathway. The 9 kD granulysin is a chemoattractant for T lymphocytes, monocytes, and other inflammatory cells and activates the expression of a number of cytokines, including RANTES, MCP-1, MCP-3, MIP-1α, IL-1, IL-6, IL-10 and IFN-α.

High serum levels of granulysin are associated with cytotoxic T cell and NK cell activity, and may thus represent a soluble biomarker for host-cellular immune responses. Serum granulysin has also been proposed as a biomarker for the Th1/Th2 balance. At the cellular level, granulysin may serve as a favorable prognostic marker for certain types of cancer.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Recombinant 9 kD granulysin
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

ELISA Detection - Quality tested
Neut, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. A concentration of 1 µg/µl of the detection antibody was utilized to gernerate the example standard curve. It is recommended that the end-user titrate each lot of reagents for optimal performance.

Application Notes

ELISA Detection: To measure human granulysin, this antibody can be used as a detection antibody in sandwich ELISA format and paired with the purified DH2 antibody (Cat. No. 348007) as the capture antibody.
Additional reported applications (for the relevant formats) include: neutralization1 and immunoprecipitation1.
Note: For testing human granulysin in serum, plasma or cell culture supernatant, LEGEND MAX™ Human Granulysin ELISA Kit with Pre-coated Plates (Cat. No. 438007 & 438008) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Tewari P, et al. 2010. Blood. 116:3465. (Neut, IP)
AB_2563603 (BioLegend Cat. No. 526103)
AB_2563604 (BioLegend Cat. No. 526104)

Antigen Details

Saposin-like protein family of lipid binding protein, 9 kD and 15 kD

NK cells, cytolytic T cells

Cytolytic against bacteria, fungi, parasite and tumor cells; chemoattract inflammatory cells
Cell Type
Neutrophils, NK cells, T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family
Antigen References

1. Hanson DA, et al. 1999. Mol. Immunol. 36:413.
2. Spada FM, et al. 2000. J. Exp. Med. 191:937.
3. Kalb ML, et al. 2012. J. Immunol. 188:1583.

Gene ID
10578 View all products for this Gene ID
View information about Granulysin on

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 06.13.2014

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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  • Biotin anti-human Granulysin


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