Biotin anti-human Granulysin Antibody

Pricing & Availability
Clone
DH10 (See other available formats)
Other Names
GNLY, 519, LAG2, NKG5, LAG-2, D2S69E, TLA519
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
526103 50 µg 120€
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526104 500 µg 396€
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Description

Granulysin is a cytolytic protein present in granules of human natural killer (NK) cells and activated cytotoxic T lymphocytes (CTLs). Granulysin is synthesized as a 15 kD precursor, which is then cleaved at both the amino- and carboxy-termini into a 9 kD activated form.

The 9 kD granulysin is released into the intercellular space between target and effector cells via a granule exocytosis pathway. The 9 kD granulysin is a chemoattractant for T lymphocytes, monocytes, and other inflammatory cells and activates the expression of a number of cytokines, including RANTES, MCP-1, MCP-3, MIP-1α, IL-1, IL-6, IL-10 and IFN-α.

High serum levels of granulysin are associated with cytotoxic T cell and NK cell activity, and may thus represent a soluble biomarker for host-cellular immune responses. Serum granulysin has also been proposed as a biomarker for the Th1/Th2 balance. At the cellular level, granulysin may serve as a favorable prognostic marker for certain types of cancer.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant 9 kD granulysin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested
Neut, IP - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. A concentration of 1 µg/µl of the detection antibody was utilized to gernerate the example standard curve. It is recommended that the end-user titrate each lot of reagents for optimal performance.

Application Notes

ELISA Detection: To measure human granulysin, this antibody can be used as a detection antibody in sandwich ELISA format and paired with the purified DH2 antibody (Cat. No. 348007) as the capture antibody. Recombinant Human Granulysin (ELISA Std.) (Cat. No. 552109) can be used as the protein standard.
Additional reported applications (for the relevant formats) include: neutralization1 and immunoprecipitation1.
Note:
For testing human granulysin in serum, plasma or cell culture supernatant, LEGEND MAX™ Human Granulysin ELISA Kit with Pre-coated Plates (Cat. No. 438007 & 438008) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Tewari P, et al. 2010. Blood. 116:3465. (Neut, IP)
RRID
AB_2563603 (BioLegend Cat. No. 526103)
AB_2563604 (BioLegend Cat. No. 526104)

Antigen Details

Structure
Saposin-like protein family of lipid binding protein, 9 kD and 15 kD
Distribution
NK cells, cytolytic T cells
Function
Cytolytic against bacteria, fungi, parasite and tumor cells; chemoattract inflammatory cells
Cell Type
NK cells, T cells, Neutrophils
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Hanson DA, et al. 1999. Mol. Immunol. 36:413.
2. Spada FM, et al. 2000. J. Exp. Med. 191:937.
3. Kalb ML, et al. 2012. J. Immunol. 188:1583.

Gene ID
10578 View all products for this Gene ID
UniProt
View information about Granulysin on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 06.13.2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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