APC anti-human CD19 Antibody

Pricing & Availability
Clone
HIB19 (See other available formats)
Regulatory Status
RUO
Workshop
V CD19.11
Other Names
B4
Isotype
Mouse IgG1, κ
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Product Citations
publications
HIB19_APC_061807
Human peripheral blood lymphocytes stained with HIB19 APC
  • HIB19_APC_061807
    Human peripheral blood lymphocytes stained with HIB19 APC
See APC spectral data
Cat # Size Price Quantity Avail. Save
302211 25 tests 32,00€
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302212 100 tests 76,00€
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Description

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Chimpanzee, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The CD19 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections8 and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302267 & 302268).

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Bradbury L, et al. 1993. J. Immunol. 151:2915.
  4. Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
  5. Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
  6. Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Audigé A, et al. 2017. BMC Immunology. 10.1186/s12865-017-0209-9. PubMed
  2. Nakachi S, et al. 2017. Arthritis Research & Therapy . 10.1186/s13075-017-1309-x. PubMed
  3. den Hartog G, et al. 2018. Eur J Immunol. 48:283. PubMed
  4. Yarzabek B et al. 2018. eLife. 7 pii: e34961. PubMed
  5. Ivan Jelcic et al. 2018. Cell. 175(1):85-100 . PubMed
  6. Rochat MA, et al. 2018. Virol J. 15:191. PubMed
  7. Xu L, et al. 2019. Haematologica. 10.3324/haematol.2018.207258. PubMed
  8. Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed
  9. Nelde A, et al. 2018. Oncoimmunology. 7:e1316438. PubMed
  10. Guan J, et al. 2018. Mol Cancer Ther. 17:1090. PubMed
  11. Velázquez–Avila M, et al. 2019. Leukemia. 33:1337. PubMed
  12. Wu Y, et al. 2019. Nat Med. 25:776. PubMed
  13. Messinger JE, et al. 2019. MBio. 10. PubMed
  14. Benci JL et al. 2019. Cell. 178(4):933-948 . PubMed
  15. Roybal KT et al. 2016. Cell. 167(2):419-432 . PubMed
  16. Linedale R, et al. 2017. PLoS One. 12:e0175755. PubMed
  17. Lee HJ, et al. 2017. Oncotarget. 8:113345. PubMed
  18. Gärtner K, et al. 2019. J Extracell Vesicles. 8:1573051. PubMed
  19. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  20. Shi Z, et al. 2019. Cell Discov. 0.252777778. PubMed
  21. Weisberg SP, et al. 2020. Cell Reports. 29(12):3916-3932.e5.. PubMed
  22. Rendeiro AF, et al. 2020. Nat Commun. 11:577. PubMed
  23. Yamaguchi K, et al. 2018. Cancer Sci. 109:3032. PubMed
  24. Chabi S, et al. 2020. Cell Reports. 29(8):2307-2320.e6.. PubMed
  25. Lavaert M, et al. 2020. Immunity. 52(6):1088-1104. PubMed
  26. Urlaub D, et al. 2019. Arthritis Res Ther. 1.067361111. PubMed
  27. Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed
  28. Bhattacharya S, et al. 2020. Front Bioeng Biotechnol. 1.055555556. PubMed
  29. Moreno I, et al. 2010. PLoS One. 5:e743. PubMed
  30. Riboldi E, et al. 2011. J Biol Chem. 286:35329. PubMed
  31. Guo X, et al. 2016. Mol Ther Methods Clin Dev. 3:15054. PubMed
  32. Mohiuddin M, et al. 2016. Nat Commun. 7: 11138. PubMed
  33. S Tzeng 2016. J Vis Exp. 118:e54582. PubMed
  34. Aagaard K, et al. 2017. Sci Rep. 7:41389. PubMed
  35. Veras FP, et al. 2020. J Exp Med. 217:00:00. PubMed
  36. Lan X, et al. 2020. Molecular Cell. 81(2):239-254.e8. PubMed
  37. Dai Q, et al. 2020. Front Immunol. 11:539654. PubMed
  38. Evers M, et al. 2020. MAbs. 12:1795505. PubMed
  39. Zeng J, et al. 2020. Nat Med. 26:535. PubMed
  40. Kang L, et al. 2020. Exp Hematol Oncol. 9:11. PubMed
  41. Constantinescu-Bercu A, et al. 2020. eLife. 9:00. PubMed
  42. Richardson SE, et al. 2021. STAR Protocols. 2(2):100420. PubMed
RRID
AB_314241 (BioLegend Cat. No. 302211)
AB_314242 (BioLegend Cat. No. 302212)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution

B lineage (except plasma cells), follicular dendritic cells

Function
B cell activation and differentiation
Ligand/Receptor
Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.

Gene ID
930 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12.18.2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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