- NT73 (See other available formats)
- Regulatory Status
- Other Names
- Softag 1
NeoClone Catalog# S0001
- Mouse IgG1
- Ave. Rating
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- Product Citations
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E. coli RNA Core polymerase is useful in studying mechanisms of transcription initiation. E. coli Core polymerase purified with Softag 4 may be free of sigma factors found in holoenzyme.Product Details
- Verified Reactivity
- E. coli strain MG1655, Enterics, Borellia burgdorferi, Salmonella
- Antibody Type
- Host Species
- 50% anti- E. coli RNA Polymerase antibody conjugated resin is supplied in 1X PBS and 0.09% NaN3. The volume specified for each catalog number indicates the volume of resin included.
- Storage & Handling
- Store at 4°C until use. Use at room temperature.
IP - Quality tested
- Recommended Usage
Resin binding capacity is greater than 0.15 mg/mL (based on 28kD tagged protein). To elute, use TE, 0.7M NaCl, 30% propylene glycol.
- Application Notes
This resin is also appropriate for use in identifying proteins associated with the recombinant protein tagged with Softag 1. Softag 1 is an immunoaffinity system appropriate for purifying tagged proteins from eukaryotic expression sources. The epitope (SLAELLNAGLGGS) is derived from a subunit of prokaryotic RNAP.
(PubMed link indicates BioLegend citation)
Previously item# S0001 from Neoclone.
- Thompson NE, et al. 2003. Anal. Biochem. 323:171.
- Biology Area
- Cell Biology
- Molecular Family
- Nuclear Markers
- Gene ID
- 948488 View all products for this Gene ID
- View information about RNA Polymerase ImmunoAffinity Resin on UniProt.org
|Anti-E. coli RNA Polymerase ImmunoAffinity Resin||NT73||IP|
|Purified anti-E. coli RNA Polymerase β Prime||NT73||WB, ELISA, IP|
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