AEC Substrate Buffer

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Substrate Buffer
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925903 50 mL 24€
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The AEC solution reacts to give a red staining with minimal background noise.

Product Details
Technical data sheet

Product Details

Storage & Handling
Store this product between 2 and 8°C. AEC preparations should be stored in a manner which minimizes exposure to light.


Recommended Usage

Each lot of this product is quality control tested by immunohistochemistry.

AEC Substrate Buffer is for use with AEC chromogen concentrate (Cat. No. 925804). The optimal working dilution should be determined for each specific assay condition.

Application Notes

AEC reaction product is soluble in alcohol. Do not use organic solvents after AEC staining.

1. Dilute AEC concentrate (Cat. No. 925804) 1:50 with Substrate Buffer
2. Apply mixture to tissue section
3. Incubate the slides for 5-10 min. at room temperature
4. Rinse tissue sections with deionized water
5. Counterstain and mount using aqueous mounting media
6. AEC induces a red precipitate with almost no background

Antigen Details

Gene ID

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For Research Use Only. Not for diagnostic or therapeutic use.


This product is supplied subject to the terms and conditions, including the limited license, located at ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.


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