- HM48-1 (See other available formats)
- Regulatory Status
- Other Names
- BCM1, SLAMF2, Blast-1
- Armenian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
CD48 is a 45 kD GPI-anchored glycoprotein also known as BCM1, Blast-1 (human), and OX-45 (rat). It is a member of the Ig superfamily, expressed on T and B cells and monocytes/macrophages. It plays a role in adhesion and T cell recognition. The primary ligands for CD48 are CD2 and CD244.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Armenian Hamster
- Mouse T lymphoma MBL-2
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The HM48-1 antibody is useful for blocking in vitro and in vivo CD48 mediated interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation1, costimulation of T cell proliferation1,2, blocking of CD48-CD2 interaction1, and inhibition of CTL activity and graft rejection1,2. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. Nos. 103452-103456) with a lower endotoxin limit (Endotoxin < 0.01 EU/µg).
(PubMed link indicates BioLegend citation)
- Kato K, et al. 1992. J. Exp. Med. 176:1241. (IP, Costim, Block)
- Qin L, et al. 1994. J. Exp. Med. 179:341. (Costim, Block)
AB_2832311 (BioLegend Cat. No. 103455)
AB_2832312 (BioLegend Cat. No. 103456)
AB_2832310 (BioLegend Cat. No. 103454)
AB_2832308 (BioLegend Cat. No. 103452)
AB_2832309 (BioLegend Cat. No. 103453)
- Ig superfamily, 45 kD
T cells and B cells, monocytes/macrophages
- Adhesion, T cell recognition
- CD2, CD244
- Cell Type
- B cells, Macrophages, Monocytes, T cells
- Biology Area
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Flament C, et al. 1996. Hum. Immunol. 46:82.
3. Van der Merwe PA, et al. 1995. Curr. Biol. 5:74.
4. Latchman Y, et al. 1998. J. Immunol. 161:5809.
- Gene ID
- 12506 View all products for this Gene ID
- View information about CD48 on UniProt.org
- Do you guarantee that your antibodies are totally pathogen free?
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-mouse CD48
PE anti-mouse CD48
Purified anti-mouse CD48
Biotin anti-mouse CD48
APC anti-mouse CD48
Alexa Fluor® 488 anti-mouse CD48
Alexa Fluor® 647 anti-mouse CD48
Pacific Blue™ anti-mouse CD48
PE/Cyanine5 anti-mouse CD48
PerCP/Cyanine5.5 anti-mouse CD48
PE/Cyanine7 anti-mouse CD48
Alexa Fluor® 700 anti-mouse CD48
Brilliant Violet 421™ anti-mouse CD48
APC/Cyanine7 anti-mouse CD48
Purified anti-mouse CD48 (Maxpar® Ready)
Alexa Fluor® 594 anti-mouse CD48
Brilliant Violet 605™ anti-mouse CD48
Brilliant Violet 510™ anti-mouse CD48
Brilliant Violet 711™ anti-mouse CD48
PE/Dazzle™ 594 anti-mouse CD48
APC/Fire™ 750 anti-mouse CD48
TotalSeq™-A0429 anti-mouse CD48
Brilliant Violet 785™ anti-mouse CD48
Ultra-LEAF™ Purified anti-mouse CD48
TotalSeq™-B0429 anti-mouse CD48
TotalSeq™-C0429 anti-mouse CD48