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- Clone
- AD2 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- V B-CD73.3
- Other Names
- Ecto-5'-nucleotidase, E.C3.1.3.5, L-VAP-2, NT5E, 5'-NT
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
CD73 is a 70 kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase, which is also known as ecto-5'-nucleotidase. It converts adenosine monophosphate (AMP) to adenosine. CD73 is expressed on subsets of T and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells. It has been reported that CD73 costimulates T cell activation, and mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC
- Recommended Usage
-
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include:immunofluorescence3.
Clone AD2 has been noted to induce clustering and internalization of CD73 in vivo and inhibit metastasis in a murine breast cancer xenograft model4. - Additional Product Notes
-
For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
-
Application References
(PubMed link indicates BioLegend citation) -
- Nakamura T, et al. 1993. J. Immunol. 151:6933.
- Liao J, et al. 2011. J Endod. 37:1217. PubMed
- Touboul C, et al. 2013. J. Transl. Med. 11:28. (IF)
- Terp MG, et al. 2013. J Immunol. 191: 4165-73 (Block)
Antigen Details
- Structure
- GPI-linked 5'-nucleotidase, 70 kD
- Distribution
-
Subsets of T cells and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells
- Function
- Catalyses dephosphorylation of adenosine monophosphate, costimulates T cell activation, mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Epithelial cells, Mesenchymal Stem Cells, T cells, Tregs
- Biology Area
- Costimulatory Molecules, Immunology, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Zola H, et al. 2007. Leukocyte and stromal Cell Molecules:the CD Markers. A John Wiley & Sons Inc, Publication.
2. Airas L and Jalkanen S, et al. 1996. Blood 88:1755.
3. Gutensohn W, et al. 1995. Cell Immunol. 161:213.
4. Airas L, et al. 1995. J. Exp. Med. 182:1603. - Gene ID
- 4907 View all products for this Gene ID
- UniProt
- View information about CD73 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- What are Flexi-Fluors?
-
Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.
- How quickly will I receive my order?
-
We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.
- How are Flexi-Fluors different from regular catalog products?
-
Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.
- How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?
-
Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
- I can’t find the antibody-dye combination that I need. When will it be available?
-
We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.
- I need help to validate the performance of my Flexi-Fluor. Who should I contact?
-
Please get in touch with Technical Service for assistance.
- Can I order more than 50 μg of a Flexi-Fluor?
-
Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.
- What is the expiration date of my Flexi-Fluor?
-
Expiration dates can be found on the vial label or by using our CoA lookup tool.
Other Formats
View All CD73 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-human CD73 (Ecto-5'-nucleotidase)
Human peripheral blood lymphocytes were stained with CD3 APC... 
-
Brilliant Violet 421™ anti-human CD73 (Ecto-5'-nucleotidase)
Human peripheral blood lymphocytes were stained with CD3 FIT... 
Frozen human tonsil section was fixed with 4% paraformaldehy... -
Purified anti-human CD73 (Ecto-5'-nucleotidase)
Human peripheral blood lymphocytes stained with AD2 PE and C... 
Human peripheral blood lymphocytes stained with AD2 PE and C... 
Frozen human tonsil section was fixed with 4% paraformaldehy... -
PE anti-human CD73 (Ecto-5'-nucleotidase)
Human peripheral blood lymphocytes stained with AD2 PE and C...
Human peripheral blood lymphocytes stained with AD2 PE and C... -
APC anti-human CD73 (Ecto-5'-nucleotidase)
Human peripheral blood lymphocytes stained with AD2 APC and ... 
-
PE/Cyanine7 anti-human CD73 (Ecto-5'-nucleotidase)
Human peripheral blood lymphocytes were stained with CD3 Pac... -
Pacific Blue™ anti-human CD73 (Ecto-5'-nucleotidase)
Human peripheral blood lymphocytes were stained with CD3 FIT... 
-
PerCP/Cyanine5.5 anti-human CD73 (Ecto-5'-nucleotidase)
Human peripheral blood lymphocytes were stained with CD3 APC... -
Biotin anti-human CD73 (Ecto-5'-nucleotidase)
Human peripheral blood lymphocytes were stained CD3 APC and ... 
-
PE/Dazzle™ 594 anti-human CD73 (Ecto-5'-nucleotidase)
Human peripheral blood lymphocytes were stained with CD3 APC... 
-
APC/Cyanine7 anti-human CD73 (Ecto-5'-nucleotidase)
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Brilliant Violet 605™ anti-human CD73 (Ecto-5'-nucleotidase)
Human peripheral blood lymphocytes were stained with CD3 FIT... 
-
Brilliant Violet 711™ anti-human CD73 (Ecto-5'-nucleotidase)
Human peripheral blood lymphocytes were stained with CD3 PE ... 
-
Brilliant Violet 785™ anti-human CD73 (Ecto-5'-nucleotidase)
Human peripheral blood lymphocytes were stained with CD3 PE ... 
-
TotalSeq™-A0577 anti-human CD73 (Ecto-5'-nucleotidase)
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TotalSeq™-C0577 anti-human CD73 (Ecto-5'-nucleotidase)
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TotalSeq™-B0577 anti-human CD73 (Ecto-5'-nucleotidase)
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APC/Fire™ 750 anti-human CD73 (Ecto-5'-nucleotidase)
Human peripheral blood lymphocytes were stained with anti-hu... -
TotalSeq™-D0577 anti-human CD73 (Ecto-5'-nucleotidase)
-
Alexa Fluor® 700 anti-human CD73 (Ecto-5'-nucleotidase)
Human peripheral blood lymphocytes were stained with anti-hu... -
Alexa Fluor® 647 anti-human CD73 (Ecto-5'-nucleotidase)
Human peripheral blood lymphocytes were stained with anti-hu... -
Brilliant Violet 510™ anti-human CD73 (Ecto-5'-nucleotidase)
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP/Fire™ 780 anti-human CD73 (Ecto-5'-nucleotidase)
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Red™ 718 anti-hu CD73 (Ecto-5'-nucleotidase) (Flexi-Fluor™)
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