- Clone
- AD2 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- V B-CD73.3
- Other Names
- Ecto-5'-nucleotidase, E.C3.1.3.5, L-VAP-2, NT5E, 5'-NT
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood lymphocytes stained with AD2 APC and CD19 PE (top) or mouse IgG1, k APC isotype control and CD19 PE (bottom). -
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 344005 | 25 tests | 112,00€ | ||||
| 344006 | 100 tests | 236,00€ | ||||
CD73 is a 70 kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase, which is also known as ecto-5'-nucleotidase. It converts adenosine monophosphate (AMP) to adenosine. CD73 is expressed on subsets of T and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells. It has been reported that CD73 costimulates T cell activation, and mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells.
Product DetailsProduct Details
- Reactivity
- Human, African Green, Baboon
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
- Concentration
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include:immunofluorescence3.
clone AD2 has been noted to induce clustering and internalization of CD73 in vivo and inhibit metastasis in a murine breast cancer xenograft model4. -
Application References
(PubMed link indicates BioLegend citation) -
- Nakamura T, et al. 1993. J. Immunol. 151:6933.
- Liao J, et al. 2011. J Endod. 37:1217. PubMed
- Touboul C, et al. 2013. J. Transl. Med. 11:28. (IF)
- Terp MG, et al. 2013. J Immunol. 191: 4165-73 (Block)
- Product Citations
- RRID
-
AB_1877158 (BioLegend Cat. No. 344005)
AB_1877157 (BioLegend Cat. No. 344006)
Antigen Details
- Structure
- GPI-linked 5'-nucleotidase, 70 kD
- Distribution
-
Subsets of T cells and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells
- Function
- Catalyses dephosphorylation of adenosine monophosphate, costimulates T cell activation, mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Epithelial cells, Mesenchymal Stem Cells, T cells, Tregs
- Biology Area
- Costimulatory Molecules, Immunology, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Zola H, et al. 2007. Leukocyte and stromal Cell Molecules:the CD Markers. A John Wiley & Sons Inc, Publication.
2. Airas L and Jalkanen S, et al. 1996. Blood 88:1755.
3. Gutensohn W, et al. 1995. Cell Immunol. 161:213.
4. Airas L, et al. 1995. J. Exp. Med. 182:1603. - Gene ID
- 4907 View all products for this Gene ID
- UniProt
- View information about CD73 on UniProt.org
Related Pages & Pathways
Pathways
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