Purified anti-Myelin Protein Zero Antibody

Pricing & Availability
Clone
58A (See other available formats)
Regulatory Status
RUO
Other Names
MPZ, Myelin Protein Zero
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
58A_Purified_Myelin_Protein_Zero_Antibody_1_040219.png
IHC staining of purified anti-Myelin Protein Zero antibody (clone 58A) on formalin-fixed paraffin-embedded mouse sciatic nerve tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 58A_Purified_Myelin_Protein_Zero_Antibody_1_040219.png
    IHC staining of purified anti-Myelin Protein Zero antibody (clone 58A) on formalin-fixed paraffin-embedded mouse sciatic nerve tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 58A_Purified_Myelin_Protein_Zero_Antibody_2_040219.png
    IHC staining of purified anti-Myelin Protein Zero antibody (clone 58A) on formalin-fixed paraffin-embedded mouse sciatic nerve tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 58A_Purified_Myelin_Protein_Zero_Antibody_3_040219.png
    IHC staining of purified anti-Myelin Protein Zero antibody (clone 58A) on formalin-fixed paraffin-embedded human dorsal root ganglia tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 58A_Purified_Myelin_Protein_Zero_Antibody_4_040219.png
    Western blot of purified anti-Myelin Protein Zero antibody (clone 58A). Lane 1: Molecular weight marker; Lane 2: 20µg of mouse sciatic nerve lysate; Lane 3: 20µg of rat sciatic nerve lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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862201 25 µg 72,00€
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862202 100 µg 188,00€
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Description

Myelin protein zero (MPZ), is a membrane glycoprotein mainly expressed in the peripheral nervous system (PNS). It is the major structural component of the myelin sheath, and accounts for at least 50% of all proteins in the PNS. MPZ is expressed by Schewann cells. Mutations in MPZ have been associated with myelin deficiency, which may lead to neuropathies including charcot-Marie-Tooth and Dejerine-Sottas diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. For western blotting, the suggested use of this reagent is 1 - 5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Yoshimura K, et al. 1996. Neurosci Res. 25(1):41 (WB, IHC, ELISA)
RRID
AB_2801241 (BioLegend Cat. No. 862201)
AB_2801242 (BioLegend Cat. No. 862202)

Antigen Details

Structure
MPZ is a 248 amino acid protein with an apparent molecular mass of ~ 28 kD.
Distribution

Tissue Distribution: Peripheral nervous system
Cellular Distribution: Plasma membrane, extracellular, endoplasmic reticulum, and lysosome

Function
MPZ is an adhesion molecule involved in the formation of myelin sheath in the peripheral nervous system.
Interaction
Forms homodimer and homotetramer
Biology Area
Neurodegeneration, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules
Antigen References
  1. Chiò A and Lauria G. 2016. Lancet Neurol. 15(1):12
  2. Brennan KM, et al. 2015. Neurosci Lett. 596:14
  3. Tazir M, et al. 2014. J Neurol Sci. 347(1-2):14
Gene ID
4359 View all products for this Gene ID 17528 View all products for this Gene ID 24564 View all products for this Gene ID
UniProt
View information about Myelin Protein Zero on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 04.02.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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