Purified anti-mTOR Antibody

Pricing & Availability
Clone
6H9B10 (See other available formats)
Other Names
FKBP12-rapamycin complex-associated protein 1, FK506 binding protein 12-rapamycin associated protein 1, FRAP1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
6H9B10_PURE_mTOR_Antibody_1_WB_071519
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-mTOR Antibody, clone 6H9B10, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker. Lysates were loaded in order of descending mTOR expression (Source: Human Protein Atals).
  • 6H9B10_PURE_mTOR_Antibody_1_WB_071519
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-mTOR Antibody, clone 6H9B10, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker. Lysates were loaded in order of descending mTOR expression (Source: Human Protein Atals).
Cat # Size Price Quantity Avail. Save
659201 25 µg 60€
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659202 100 µg 164€
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Description

mTOR (mammalian target of rapamycin) is a 289 kD member of the PI3 kinase family, containing HEAT, FRB, and FATC domains. mTOR is expressed in the nucleus and cytoplasm, and is a target for cell-cycle arrest and the immunosuppressive effects of FKBP12-rapamycin complex. mTOR controls ribosome biogenesis and cell growth, and acts as an ATP sensor. mTOR is repressed by nutrient deprivation and mitochondrial uncoupling. mTOR is the target of PI3K-regulated protein kinase, AKT/PKB (phosphorylated at Thr 2446 and Ser2448). mTOR has also been shown to bind RAPTOR, which negatively regulates mTOR kinase activity, and is also inhibited by tuberin-hamartin tumor suppressor complex. The binding of RAPTOR to mTOR is necessary for the mTOR-catalyzed phosphorylation of 4EBP1 in vitro, and it strongly enhances the mTOR kinase activity toward p70-alpha.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, weakly cross-reactive with mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human mTOR recombinant protein (1186-1385 aa) expressed in E. coli.
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Sohrabi Y, et al. 2019. Front Immunol. 9:3155. PubMed
Publication Library
RRID
AB_2563155 (BioLegend Cat. No. 659201)
AB_2563156 (BioLegend Cat. No. 659202)

Antigen Details

Structure
2549 amino acids encode a protein with predicted MW of 289 kD.
Distribution

Nucleus, Cytoplasm

Function
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex.
Interaction
Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40, and DEPTOR; also part of the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PRR5, RICTOR, MAPKAP1, and DEPTOR.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Signal Transduction
Antigen References

1. Brown EJ, et al. 1994. Nature. 369:756.
2. Dennis P, et al. 2001. Science. 294:1102.
3. Fingar D, et al. 2002. Genes Dev. 16:1472.
4. Nojima H, et al. 2003. J. Biol. Chem. 278:15461.

Gene ID
2475 View all products for this Gene ID
UniProt
View information about mTOR on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 07.15.2019

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