Purified anti-human CD50 (ICAM-3) Antibody

Pricing & Availability
Clone
CBR-IC3/1 (See other available formats)
Regulatory Status
RUO
Other Names
ICAM-3
Isotype
Mouse IgG1, κ
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Product Citations
publications
CBR-IC3slash1_Pure_090808.jpg
Human peripheral blood lymphocytes stained with purified CBR-IC3/1, followed by anti-mouse IgG FITC
  • CBR-IC3slash1_Pure_090808.jpg
    Human peripheral blood lymphocytes stained with purified CBR-IC3/1, followed by anti-mouse IgG FITC
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330002 100 µg 90€
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Description

CD50 is a 120-140 kD type I membrane protein also known as intercellular adhesion molecule 3 (ICAM-3). It is broadly expressed on leukocytes, endothelial cells, and Langerhans cells. CD50 is a member of the Ig superfamily. It provides costimulatory signal for T cells and regulates cell adhesion through interaction with LFA-1 (CD11a/CD18, αL2) integrin.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SKW3 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP - Reported in the literature, not verified in house
 

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications include: immunoprecipitation1 and blocking1-3.

Application References

(PubMed link indicates BioLegend citation)
  1. deFougerolles AR and Springer TA, et al. 1992. J. Exp. Med. 175:185. (IP, Block)
  2. Geijtenbeek T, et al. 2000. Cell 100:575. (Block)
  3. Giannoni E, et al. 2003. J Biol. Chem. 278:36763. (Block)
Product Citations
  1. Sugden SM, et al. 2017. J Virol. 91:e02445. PubMed
RRID
AB_1186142 (BioLegend Cat. No. 330002)

Antigen Details

Structure
Ig superfamily, type I membrane protein, 130 kD
Distribution

Thymocytes, T and B cells, monocytes, granulocytes, endothelial cells

Function
Adhesion, costimulation
Ligand/Receptor
CD11a/CD18 (LFA-1), integrin αL2
Cell Type
B cells, Endothelial cells, Granulocytes, Monocytes, T cells, Thymocytes
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Valent P, et al. 2001. Immunol. Rev. 179:74.

Gene ID
3385 View all products for this Gene ID
UniProt
View information about CD50 on UniProt.org
Go To Top Version: 3    Revision Date: 08.25.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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