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PE anti-human CD50 (ICAM-3) Antibody

Pricing & Availability
Clone
CBR-IC3/1 (See other available formats)
Regulatory Status
RUO
Other Names
ICAM-3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
CBR-IC3slash1_PE_090808.jpg
Human peripheral blood lymphocytes stained with CBR-IC3/1 PE
  • CBR-IC3slash1_PE_090808.jpg
    Human peripheral blood lymphocytes stained with CBR-IC3/1 PE
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330005 25 tests 108€
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Description

CD50 is a 120-140 kD type I membrane protein also known as intercellular adhesion molecule 3 (ICAM-3). It is broadly expressed on leukocytes, endothelial cells, and Langerhans cells. CD50 is a member of the Ig superfamily. It provides costimulatory signal for T cells and regulates cell adhesion through interaction with LFA-1 (CD11a/CD18, αL/β2) integrin.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SKW3 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications include: immunoprecipitation1 and blocking1-3.

Application References

(PubMed link indicates BioLegend citation)
  1. deFougerolles AR and Springer TA, et al. 1992. J. Exp. Med. 175:185. (IP, Block)
  2. Geijtenbeek T, et al. 2000. Cell 100:575. (Block)
  3. Giannoni E, et al. 2003. J Biol. Chem. 278:36763. (Block)
Product Citations
  1. Gatault S, et al. 2015. J Immunol. 195: 2483-2492. PubMed
  2. Agostinis C, et al. 2019. Int J Mol Sci. 1.947222222. PubMed
RRID
AB_1186057 (BioLegend Cat. No. 330005)

Antigen Details

Structure
Ig superfamily, type I membrane protein, 130 kD
Distribution

Thymocytes, T and B cells, monocytes, granulocytes, endothelial cells

Function
Adhesion, costimulation
Ligand/Receptor
CD11a/CD18 (LFA-1), integrin αL/ß2
Cell Type
B cells, Endothelial cells, Granulocytes, Monocytes, T cells, Thymocytes
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Valent P, et al. 2001. Immunol. Rev. 179:74.

Gene ID
3385 View all products for this Gene ID
UniProt
View information about CD50 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 06.18.2013

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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