- 2E1.E9 (See other available formats)
- Regulatory Status
- Other Names
- Glial fibrillary acid protein
- Mouse IgG2b
- Ave. Rating
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- Product Citations
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GFAP is expressed exclusively in astrocytes in the central nervous system. The protein is a member of the intermediate filament family of proteins which form networks providing support and strength to cells. This antibody does not cross-react with other intermediate filaments such as vimentin, neurofilament proteins, desmin and others. More than 50 GFAP mutations have been identified to be associated with the Alexander disease.Product Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Host Species
- Bovine spinal cord homogenate
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
ICC, IHC-F, IHC-P - Verified
FC - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.25 - 1.0 µg antibody per mL antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration range of 1.0 - 5.0 µg/mL is recommended. For immunohistochemical staining of frozen tissue sections, a concentration range of 5.0 - 10 µg/mL is suggested. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
For Immunohistochemistry, Citrate buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 is recommended.
(PubMed link indicates BioLegend citation)
- McLendon RE and Bigner DD. 1994. Brain Pathol. 4:221.
- Liu W, et al. 2011. Proteomics 11:3556. (FC) PubMed
- Product Citations
AB_2109791 (BioLegend Cat. No. 644701)
AB_2294566 (BioLegend Cat. No. 644702)
- Around 50 kD
Only expressed in astrocytes in the central nervous system.
- Together with other proteins to form intermediate filaments which supports astroglial cells. Astroglial cells support and nourish cells in the brain and spinal cord. If brain cells are injured through trauma or disease, astroglial cells react by rapidly prodcing more GFAP protein.
- Cell Type
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Intermediate Filaments
- Gene ID
- 2670 View all products for this Gene ID
- View information about GFAP on UniProt.org
|Alexa Fluor® 488 anti-GFAP||2E1.E9||IHC-F,ICC|
|Alexa Fluor® 647 anti-GFAP||2E1.E9||IHC-F,IHC-P|
|Alexa Fluor® 594 anti-GFAP||2E1.E9||IHC-F|
|Brilliant Violet 421™ anti-GFAP||2E1.E9||IHC-F,ICC,IHC-P|
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