FITC anti-human CD45 Antibody

Pricing & Availability
Clone
HI30 (See other available formats)
Workshop
IV N816
Other Names
LCA, T200
Isotype
Mouse IgG1, κ
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Product Citations
publications
Hi30
Human peripheral blood lymphocytes, monocytes and granulocytes stained with HI30 FITC
  • Hi30
    Human peripheral blood lymphocytes, monocytes and granulocytes stained with HI30 FITC
See FITC spectral data
Cat # Size Price Quantity Avail. Save
304005 25 tests 24€
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304006 100 tests 52€
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304054 100 µg 96€
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304038 500 tests 236€
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Description

CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Cross-Reactivity: Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
µg sizes: 0.5 mg/ml
test sizes: lot-specific (please contact technical support for concentration and total µg amount)
Storage & Handling
The CD45 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections9, inhibition of CD45 functions4, immunofluorescence11, and Western blotting3.

It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Kishihara K, et al. 1993. Cell 74:143.
  3. Esser M, et al. 2001. J. Virol. 75:6173. (WB)
  4. Yamada T, et al. 2002. J. Biol. Chem. 277:28830.
  5. Nagano M, et al. 2007. Blood 110:151.
  6. Jiang Q, et al. 2008. Blood 112:2858. PubMed
  7. Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  10. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
  11. Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)
  12. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
  13. Breton G, et al. 2015. J Exp Med. 212:401. PubMed
  14. Marquardt N, et al. 2015. J Immunol. 6:2467. PubMed
  15. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
Product Citations
  1. Joly P, et al. 2017. PLoS One. 12(6):e0180568. PubMed
  2. Yuan Z, et al. 2018. Emerg Microbes Infect. 7:59. PubMed
  3. Yan Y, et al. 2018. JCI Insight. 3. PubMed
  4. Wu L, et al. 2018. Oncol Lett. 15:9507. PubMed
  5. Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed
  6. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed
  7. Rousset F, et al. 2018. Mol Ther Nucleic Acids. 14:351. PubMed
  8. Niu C, et al. 2017. Mol Med Rep. 16:4879. PubMed
  9. Sprouse ML, et al. 2019. Int J Mol Sci. 20:8. PubMed
  10. Vishnoi M, et al. 2019. Mol Oncol. 13(9): 1913. PubMed
  11. Xia Y, et al. 2019. Gastroenterol Res Pract. 2019:5436961. PubMed
  12. Saraiva L, et al. 2019. Cytometry B Clin Cytom. N/A. PubMed
  13. Selich A, et al. 2019. Stem Cell Reports. 13:262. PubMed
  14. Vishnoi M, et al. 2018. Cancer Res. 78:5349. PubMed
  15. Usmani SM et al. 2019. Cell host & microbe. 25(1):73-86 . PubMed
  16. Koide M, et al. 2018. Tohoku J Exp Med. 244:15:00. PubMed
  17. Lee HJ, et al. 2017. Oncotarget. 8:113345. PubMed
  18. Tomellini E, et al. 2020. Cell Reports. 28(4):1063-1073.e5.. PubMed
  19. Schulz A, et al. 2019. Cell Rep. 29:1074. PubMed
  20. Gomzikova MO, et al. 2019. Cells. 0.440972222. PubMed
  21. Haynes BA, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2168. PubMed
  22. Li Q, et al. 2019. Am J Transl Res. 10:4322. PubMed
  23. Baccelli I, et al. 2020. Cancer Cell. 36(1):84-99. PubMed
  24. Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed
  25. Chabi S, et al. 2020. Cell Reports. 29(8):2307-2320.e6.. PubMed
  26. Evans RDR, et al. 2020. Nat Commun. 3.491666667. PubMed
  27. Wang D, et al. 2019. Cell Res. 29:832. PubMed
  28. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. PubMed
  29. Hsieh J, et al. 2013. Nucleic Acids Res. 41:9753. PubMed
  30. Didigu C, et al. 2014. Blood. 123:61. PubMed
  31. Zhang B, et al. 2013. PLoS One. 8:57114. PubMed
  32. Wang L, et al. 2014. Proc Natl Acad Sci U S A. 111:3146. PubMed
  33. Skrbo N, et al. 2014. PLoS One. 9:113278. PubMed
  34. Saunders L, et al. 2015. Sci Transl Med. 7: 302ra136. PubMed
  35. Grove K, et al. 2016. PLoS One. 11: 0145961. PubMed
  36. Pabst C, et al. 2016. Blood. 127: 2018-2027. PubMed
  37. Genbacev O, et al. 2016. Hum Reprod. 31: 1300 - 1314. PubMed
  38. Yuan Z, et al. 2016. J Virol. 90: 7728 - 7739. PubMed
  39. S Tzeng 2016. J Vis Exp. 118:e54582. PubMed
RRID
AB_314393 (BioLegend Cat. No. 304005)
AB_314394 (BioLegend Cat. No. 304006)
AB_2564154 (BioLegend Cat. No. 304054)
AB_2562050 (BioLegend Cat. No. 304038)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)
Distribution

Hematopoietic cells, not expressed in circulating erythrocytes or platelets

Function
TCR and BCR mediated activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
Hematopoietic stem and progenitors, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References
  1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
  2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 08.19.2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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