PerCP/Cyanine5.5 Streptavidin

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Product Citations
Human peripheral blood lymphocytes were stained with biotinylated CD3, followed by SAV-PerCP/Cyanine5.5 (filled histogram), or directly stained with SAV-PerCP/Cyanine5.5 (open histogram).
  • Steptavadin_PerCpCyanine55_101818
    Human peripheral blood lymphocytes were stained with biotinylated CD3, followed by SAV-PerCP/Cyanine5.5 (filled histogram), or directly stained with SAV-PerCP/Cyanine5.5 (open histogram).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
405214 100 µg 200€
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Streptavidin binds to biotin with high affinity. Streptavidin-PerCP/Cyanine5.5 is useful for detecting biotinylated antibodies. The excitation of PerCP/Cyanine5.5 by 488 nm laser light induces a light emission maximum of 690 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Human, Mouse, Rat, All Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Streptavidin is conjugated with PerCP/Cyanine5.5 under optimal conditions.
0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin-PerCP/Cyanine5.5 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC, ICFC - Quality tested

Recommended Usage

Each lot of this Streptavidin-PerCP/Cyanine5.5 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the PerCP/Cyanine5.5 conjugation. For immunofluorescent staining, the recommended use of this reagent is = 0.06 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Streptavidin-PerCP/Cyanine5.5 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-PerCP/Cyanine5.5 is 110 kD and Streptavidin alone is 52 kD.

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Killeen ME, et al. 2013. J. Immunol. 190:4324. PubMed
  2. Grage-Griebenow E, et al. 2014. Mol Oncol. 8:982. PubMed
  3. Mise-Omata S, et al. 2014. Int Immunol. 26:607. PubMed
Product Citations
  1. Ng KK et al. 2018. eLife. 7 pii: e37851. PubMed
  2. Greenblatt SM, et al. 2018. Cancer Cell. 33:1111. PubMed
  3. Li X, et al. 2019. Stem Cells. 37:937. PubMed
  4. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  5. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  6. Delacher M, et al. 2020. Immunity. 52:295. PubMed
  7. Boguslawski KM, et al. 2020. Sci Adv. 6:eaax7515. PubMed

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 5    Revision Date: 06.18.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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