PE Streptavidin

Pricing & Availability
Other Names
Streptavidin-Phycoerythrin, SAv-PE
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Product Citations
publications
SAV_PE_082808.jpg
C57BL/6 splenocytes stained with 145-2C11 biotin, followed by SAV-PE
  • SAV_PE_082808.jpg
    C57BL/6 splenocytes stained with 145-2C11 biotin, followed by SAV-PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
405203 100 µg 36€
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405204 500 µg 128€
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-Phycoerythrin (PE) is useful for detecting biotinylated antibodies. The excitation of PE by 488 nm laser light induces a light emission maximum of 575 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with PE under optimal conditions. The solution is free of unconjugated PE.
Concentration
0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The streptavidin-PE solution should be stored undiluted between 2°C and 8°C, and protected from exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested

Recommended Usage

This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the PE conjugation. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Streptavidin-Phycoerythrin (PE) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-PE is 360 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)
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Product Citations
  1. Nishimoto K, et al. 2008. J Immunol. 181:4010. PubMed
  2. Niki T, et al. 2009. J Biol Chem. 284:32344. PubMed
  3. Chappaz S, et al. 2010. J Immunol. 185:3514. PubMed
  4. Scatizzi J, et al. 2012. J Immunol. 188:3307. PubMed
  5. Yamakawa N, et al. 2012. Int Immunol. 25:45. PubMed
  6. Baccala R, et al. 2012. J Immunol. 189:5976. PubMed
  7. Grevers L, et al. 2013. Ann Rheum Dis. 72:278. PubMed
  8. Woo S, et al. 2013. J Leukoc Biol. 93:363. PubMed
  9. Ashbaugh J, et al. 2013. J Immunol. 190:4525. PubMed
  10. Reading J, et al. 2013. J Immunol. 190:4542. PubMed
  11. Shibui A, et al. 2011. Exp Parasitol. 129:318. PubMed
  12. Kanno A, et al. 2013. Int Immunol. 25:413. PubMed
  13. Tsukamoto H, et al. 2013. Biochem Biophys Res Commun. 440:31. PubMed
  14. Günaydın G, et al. 2014. Vaccine. 32:470. PubMed
  15. Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed
  16. Datta S, et al. 2014. J Leukoc Biol. 95:853. PubMed
  17. He X, et al. 2014. J Immunol. 193:1314. PubMed
  18. Wang W, et al. 2014. Proc Natl Acad Sci U S A. 111:14466. PubMed
  19. Murakami Y, et al. 2014. J Immunol. 193:5208. PubMed
  20. Lucas A, et al. 2015. PLoS One. 10:117160. PubMed
  21. Kim Y, et al. 2015. PLoS One. 10:120294. PubMed
  22. Phiwpan K, et al. 2015. PLoS One. 10:128198. PubMed
  23. Matt P, et al. 2015. PLoS One. 10: 0137474. PubMed
  24. Mainini F, et al. 2015. PLoS One. 10: 0145403. PubMed
  25. Yoshikawa S, et al. 2016. Sci Rep. 6:18738. PubMed
  26. Davis Z, et al. 2016. PLoS Pathog. 12: 1005421. PubMed
  27. Eger C, et al. 2016. PLoS One. 11: 0150479. PubMed
  28. Mchedlidze T, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.20. PubMed
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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