PerCP/Cyanine5.5 anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
17A2_PerCPCyanine55_CD3_Antibody_020819_updated.png
C57BL/6 splenocytes were stained with CD45R/B220 APC and CD3 (clone 17A2) PerCP/Cyanine5.5 (left) or Rat IgG2b, ? PerCP/Cyanine5.5 isotype control (right).
  • 17A2_PerCPCyanine55_CD3_Antibody_020819_updated.png
    C57BL/6 splenocytes were stained with CD45R/B220 APC and CD3 (clone 17A2) PerCP/Cyanine5.5 (left) or Rat IgG2b, ? PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
100217 25 µg 95€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
100218 100 µg 259€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Abraham A, et al. 2019. J Clin Invest. 130:2685. PubMed
  2. Li SX, et al. 2019. PLoS Pathog. 15:e1007611. PubMed
  3. Kawabe T, et al. 2020. Nat Commun. 2.795833333. PubMed
  4. Marchelletta RR, et al. 2021. J Clin Invest. 131:. PubMed
  5. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  6. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  7. Chen Y, et al. 2020. Virol Sin. . PubMed
  8. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  9. Ye Y, et al. 2022. Nat Commun. 13:6458. PubMed
  10. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  11. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  12. Kaczanowska S, et al. 2021. Cell. 184(8):2033-2052.e21. PubMed
  13. Riley JS, et al. 2020. Blood Adv. 4:1102. PubMed
  14. Liang S, et al. 2022. Acta Pharm Sin B. 12:2494. PubMed
  15. Pessoa Rodrigues C, et al. 2021. Sci Adv. 7:. PubMed
  16. Han Y, et al. 2020. Nat Commun. 11:1776. PubMed
  17. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  18. Chen J, et al. 2022. J Nanobiotechnology. 20:283. PubMed
  19. Achmus L, et al. 2020. Front Neurol. 11:577971. PubMed
  20. Zhang S, et al. 2021. Cell. 184(8):2151-2166.e16. PubMed
  21. Eastman A, et al. 2016. J Immunol . 194: 5999-6010. PubMed
  22. Molofsky A, et al. 2013. J Exp Med. 210:535. PubMed
  23. Burrack KS et al. 2018. Immunity. 48(4):760-772 . PubMed
  24. Yoon Y, et al. 2021. Cancers (Basel). 13: . PubMed
  25. Cai B, et al. 2021. Mol Cancer. 20:165. PubMed
  26. Yang F, et al. 2020. Cancer Res. 80:3677. PubMed
  27. Jin Q, et al. 2022. Drug Des Devel Ther. 16:3297. PubMed
  28. Khan IM, et al. 2021. Immunohorizons. 5:703. PubMed
  29. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  30. Zirngibl F, et al. 2021. J Immunother Cancer. 9:. PubMed
  31. Yang K, et al. 2022. J Clin Invest. 132:. PubMed
  32. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
  33. Fulham MA, et al. 2019. Am J Physiol Cell Physiol. 317:C687. PubMed
  34. Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed
  35. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  36. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  37. Chakraborty S, et al. 2022. Sci Transl Med. . PubMed
  38. Mamedov MR, et al. 2018. Immunity. 48:350. PubMed
  39. Soutto M, et al. 2017. J Cancer. 8:2424. PubMed
  40. Wang Y, et al. 2021. Front Immunol. 12:654463. PubMed
  41. Pishesha N, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  42. Cheng B, et al. 2022. Cancer Commun (Lond). 42:17. PubMed
  43. Habib S, et al. 2018. Infect Immun. 86:e00019. PubMed
  44. Kennedy EM, et al. 2022. Nat Commun. 13:5907. PubMed
  45. Ercoli G, et al. 2022. Clin Transl Immunology. 11:e1366. PubMed
  46. Vidy A, et al. 2016. PLoS One. 11:e0165361. PubMed
  47. Vettorazzi S, et al. 2015. Nat Commun. 6: 7796. PubMed
  48. Cai Y, et al. 2019. Cell Rep. 27:3034. PubMed
  49. Fu Z, et al. 2021. Cell Res. 31:631. PubMed
  50. Colombo M, et al. 2022. iScience. 25:105042. PubMed
  51. Andrade ACDSP, et al. 2021. J Virol. 95:e0127621. PubMed
  52. Shafiei-Jahani P, et al. 2021. Nat Commun. 12:2526. PubMed
  53. Chatterjee D, et al. 2021. Cell Reports. 35(2):108996. PubMed
  54. Ballesteros I, et al. 2020. Cell. 183(5):1282-1297.e18. PubMed
  55. Tian Y, et al. 2016. J Immunol. 197: 1308 - 1321. PubMed
  56. Ren Y, et al. 2015. Sci Rep. 5: 18099. PubMed
  57. Agarwal P, et al. 2019. Cell Stem Cell. 24:769. PubMed
  58. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  59. Koelwyn GJ, et al. 2020. Nat Med. 1452:26. PubMed
  60. Qadir AS, et al. 2021. iScience. 24:103348. PubMed
  61. Zhou J, et al. 2022. Int J Oncol. 61: . PubMed
  62. Niderla-Bielinska J, et al. 2021. Int J Mol Sci. :22. PubMed
  63. Bennett FC, et al. 2018. Neuron. 98:1170. PubMed
  64. Shehata L, et al. 2019. Cell Rep. 28:3300. PubMed
  65. Karanika S, et al. 2022. Front Immunol. 13:972266. PubMed
  66. Al-Barwani F, et al. 2014. PLoS One. 9:104523. PubMed
  67. Varikuti S, et al. 2020. Br J Cancer. 122:1005. PubMed
  68. Ma Q, et al. 2022. Vaccines (Basel). 10:. PubMed
  69. Reda M, et al. 2022. Nat Commun. 13:4261. PubMed
  70. Zheng Y, et al. 2022. J Immunol. 208:501. PubMed
  71. Si J, et al. 2020. Cancer Cell. 38(4):551-566.e11. PubMed
  72. Laubreton D, et al. 2020. Viruses. 12:00. PubMed
  73. Mairhofer D, et al. 2015. J Invest Dermatol. 135: 2785-93. PubMed
  74. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
  75. Theurich S et al. 2017. Cell metabolism. 26(1):171-184 . PubMed
  76. Jee JJ, et al. 2022. Nat Commun. 13:18. PubMed
  77. Kim S, et al. 2022. Cancers (Basel). 14:. PubMed
  78. Tähtinen S, et al. 2022. Nat Immunol. 23:532. PubMed
  79. Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed
  80. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  81. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  82. Ukidve A, et al. 2020. Proc Natl Acad Sci U S A. 117:17727. PubMed
  83. Jiang Y, et al. 2015. MBio. 6: e01426-15. PubMed
  84. Konishi Y, et al. 2018. iScience. 10:98. PubMed
  85. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  86. Agarwal P, et al. 2021. Cell Reports. 36(2):109386. PubMed
  87. Pessoa Rodrigues C, et al. 2020. Sci Adv. 6:eaaz4815. PubMed
  88. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  89. Riopel M, et al. 2019. Mol Metab. 20:89. PubMed
  90. Song X, et al. 2022. Transl Oncol. 15:101306. PubMed
  91. Chirivi RGS, et al. 2020. Cell Mol Immunol. . PubMed
RRID
AB_1595597 (BioLegend Cat. No. 100217)
AB_1595492 (BioLegend Cat. No. 100218)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-mouse CD3 17A2 FC
PE anti-mouse CD3 17A2 FC
Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Alexa Fluor® 647 anti-mouse CD3 17A2 FC,IHC-F,3D IHC,SB
Alexa Fluor® 488 anti-mouse CD3 17A2 FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse CD3 17A2 FC
Alexa Fluor® 700 anti-mouse CD3 17A2 FC
PerCP/Cyanine5.5 anti-mouse CD3 17A2 FC
PE/Cyanine7 anti-mouse CD3 17A2 FC
APC/Cyanine7 anti-mouse CD3 17A2 FC
Brilliant Violet 421™ anti-mouse CD3 17A2 FC,ICC
Brilliant Violet 570™ anti-mouse CD3 17A2 FC
Brilliant Violet 650™ anti-mouse CD3 17A2 FC
Brilliant Violet 785™ anti-mouse CD3 17A2 FC
Brilliant Violet 510™ anti-mouse CD3 17A2 FC
APC anti-mouse CD3 17A2 FC
Ultra-LEAF™ Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Brilliant Violet 605™ anti-mouse CD3 17A2 FC
Alexa Fluor® 594 anti-mouse CD3 17A2 IHC-F,FC,3D IHC,SB
Brilliant Violet 711™ anti-mouse CD3 17A2 FC
Biotin anti-mouse CD3 17A2 FC,IHC-F
PE/Dazzle™ 594 anti-mouse CD3 17A2 FC
APC/Fire™ 750 anti-mouse CD3 17A2 FC
Brilliant Violet 750™ anti-mouse CD3 17A2 FC
TotalSeq™-A0182 anti-mouse CD3 17A2 PG
TotalSeq™-B0182 anti-mouse CD3 17A2 PG
Spark Blue™ 550 anti-mouse CD3 17A2 FC
Spark NIR™ 685 anti-mouse CD3 17A2 FC
TotalSeq™-C0182 anti-mouse CD3 17A2 PG
APC/Fire™ 810 anti-mouse CD3 17A2 FC
PE/Fire™ 640 anti-mouse CD3 17A2 FC
Spark YG™ 570 anti-mouse CD3 17A2 IHC-F
PE/Fire™ 700 anti-mouse CD3 17A2 FC
PE/Cyanine5 anti-mouse CD3 17A2 FC
Spark Blue™ 574 anti-mouse CD3 Antibody 17A2 FC
Spark Violet™ 423 anti-mouse CD3 17A2 FC
PE/Fire™ 810 anti-mouse CD3 17A2 FC
Spark Red™ 718 anti-mouse CD3 17A2 FC
Spark UV™ 387 anti-mouse CD3 17A2 FC
Go To Top Version: 3    Revision Date: 02.08.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account