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LEAF™ Purified anti-human CD218a (IL-18Rα) Antibody

Pricing & Availability
Clone
W19242A (See other available formats)
Regulatory Status
RUO
Other Names
IL-18 receptor alpha chain, IL-18 receptor 1, IL-18 receptor related protein, CDw218a
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W19242A_LEAF_CD218a_Antibody_022324
Recombinant human IL-18 (Cat. No. 592104) (black circles) stimulates the production of human IFN-γ (Cat. No. 430104) in human KG-1 cells. LEAF™ purified anti-human CD218a (IL-18Rα) (clone W19242A) (purple squares) inhibits this effect of recombinant human IL-18 (Cat. No. 592104) at 8 ng/mL in KG-1 cells in a dose-dependent manner, whereas the GoInVivo™ purified rat IgG2a, κ isotype control (Cat. No. 400562) (gray triangles) does not have this effect. ND50 range: 1-7 ng/mL.
  • W19242A_LEAF_CD218a_Antibody_022324
    Recombinant human IL-18 (Cat. No. 592104) (black circles) stimulates the production of human IFN-γ (Cat. No. 430104) in human KG-1 cells. LEAF™ purified anti-human CD218a (IL-18Rα) (clone W19242A) (purple squares) inhibits this effect of recombinant human IL-18 (Cat. No. 592104) at 8 ng/mL in KG-1 cells in a dose-dependent manner, whereas the GoInVivo™ purified rat IgG2a, κ isotype control (Cat. No. 400562) (gray triangles) does not have this effect. ND50 range: 1-7 ng/mL.
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601253 100 µg 344€
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601254 1 mg 892€
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Description

IL-18 is essential for both innate and adaptive immunity. The IL-18 receptor is composed of an α and a β subunit that form a high affinity heterodimer for IL-18. IL-18R1, also known as IL-18Rα chain and CD218a, is a type I transmembrane protein. It is expressed on NK cells, neutrophils, endothelial cells, and subsets of T and B cells. The expression of IL-18R1 and IL-18RAcP (IL-18Rβ) on lymphocytes are upregulated upon IL-12 stimulation. IL-23 and IL-18 synergistically enhance IL-18R1 expression on NK-like T cells. Upon IL-18 engagement, the intracellular TIR domain of IL-18R1 and IL-18RAcP recruits adaptor proteins, MyD88, and in turn recruits IRAK and TRAF6, activating NF-κB and MAPK pathways, which upregulates production of various inflammatory cytokines. The interaction of IL-18 and IL-18 receptor has been reported to be implicated in promotion of Th1 cytokine production and atherogenesis. IL-37 also binds to IL-18R1, but recruits IL-1R8 (SIGIRR) to activate anti-inflammatory responses.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human CD218a (IL-18Rα)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.1 EU/μg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralizing the production of human IFN-γ induced by recombinant human IL-18 at 8 ng/mL in KG-1 cells. ND50 range: 1-7 ng/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Disulfide-linked homodimer
Distribution

Expressed on NK cells and neutrophils, IL-12 activated T and B cells, endothelial cells, and smooth muscle cells; highly expressed on Hodgkin's disease cell lines

Function
IL-18Rα forms a receptor complex with IL-18Rβ and mediates IL-18 function
Interaction
Human IL-18Rα inhibits the induction of IFN-γ by human IL-18 in human KG-1 cells
Ligand/Receptor
IL-18
Cell Type
B cells, Endothelial cells, Neutrophils, NK cells, T cells
Biology Area
Adaptive Immunity, Immunology, Signal Transduction
Molecular Family
Cytokine/Chemokine Receptors
Antigen References
  1. Torigoe K, et al. 1997. J Biol Chem. 272:25737-42.
  2. Born TL, et al. 1998. J Biol Chem. 273:29445-50.
  3. Airoldi I, et al. 2000. J Immunol. 165:6880-8.
  4. van de Wetering D, et al. 2008. Int Immunol. 21:145-53.
  5. Adachi O, et al. 1998. Immunity. 9:143-50.
  6. Tsutsumi N, et al. 2014. Nat Commun. 5:5340.
Gene ID
8809 View all products for this Gene ID
UniProt
View information about CD218a on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 02.23.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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