Biotin anti-mouse CD278 (ICOS) Antibody

Pricing & Availability
Clone
7E.17G9 (See other available formats)
Other Names
Inducible COStimulatory molecule, H4
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
7Edot17G9_Biotin_081409.jpg
Con A-stimulated (3 days) Balb/c splenocytes stained with biotinylated 7E.17G9, followed by Sav-PE
  • 7Edot17G9_Biotin_081409.jpg
    Con A-stimulated (3 days) Balb/c splenocytes stained with biotinylated 7E.17G9, followed by Sav-PE
Cat # Size Price Quantity Avail. Save
117403 50 µg 64€
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Description

The 7E.17G9 antibody reacts with the 47-57 kD ICOS protein, also known as inducible costimulatory molecule, and H4. This protein is homologous to the CD28/CTLA-4 proteins. ICOS is expressed on activated T cells and a subset of thymocytes and can costimulate T cells and induce proliferation. In addition ICOS has been shown to be involved in humoral immune responses (B cell germinal center formation). The ICOS ligand, B7h/B7RP-1 and B7-H2 is constitutively expressed in B cell areas of secondary lymphoid organs and can be induced in other tissues by LPS. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development. ICOS stimulation has been shown to be involved in airway tolerance and the downregulation of pulmonary inflammation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse ICOS cDNA and ICOS hexahistidine fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume.

Application Notes

Additional reported applications (for the relevant formats) include: blocking of ligand binding. The LEAF™ format is suggested for blocking studies.

Application References

(PubMed link indicates BioLegend citation)
  1. Akbari O, et al. 2002. Nat. Med. 8:1024.
  2. Harada H, et al. 2003. J. Clin. Invest. 112:234.
  3. McAdam AJ, et al. 2000. J. Immunol. 165:5035. (FC Block)
  4. Tan SL, et al. 2006. J. Immunol. 176:2872. PubMed
Product Citations
  1. Cook KD, et al. 2015. Immunity. 43:703-714. PubMed
  2. Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
  3. Cortes JR 2018. Cancer cell. 33:259. PubMed
Publication Library
RRID
AB_961239 (BioLegend Cat. No. 117403)

Antigen Details

Structure
CD28/CTLA-4, 47-57kD
Distribution
Activated T cells, subset of thymocytes
Function
Costimulates T cell activation, proliferation, humoral immune response
Ligand/Receptor
B7h/B7RP-1/GL-50
Cell Type
T cells, Thymocytes
Biology Area
Immunology, Costimulatory Molecules
Molecular Family
CD Molecules
Antigen References

1. Rudd CE, et al. 2003. Nat. Rev. Immunol. 3:544.
2. McAdam AJ, et al. 2000. J. Immunol. 165:5035.
3. Mak TW, et al. 2003. Nat. Immunol. 4:765

Gene ID
54167 View all products for this Gene ID
UniProt
View information about CD278 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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