APC anti-T-bet Antibody

Pricing & Availability
Clone
4B10 (See other available formats)
Regulatory Status
RUO
Other Names
T-box expressed in T cells, T box 21, TBLYM
Isotype
Mouse IgG1, κ
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Product Citations
publications
4B10_APC_T-Bet_Antibody_ICFC_1_021715
Human peripheral blood lymphocytes were surface stained with CD3 PE and then treated with True-Nuclear™ Transcription Factor Buffer Set (Cat# 424401). Cells were then stained with T-bet (clone 4B10) APC (top) or mouse IgG1, κ APC isotype control (bottom).
  • 4B10_APC_T-Bet_Antibody_ICFC_1_021715
    Human peripheral blood lymphocytes were surface stained with CD3 PE and then treated with True-Nuclear™ Transcription Factor Buffer Set (Cat# 424401). Cells were then stained with T-bet (clone 4B10) APC (top) or mouse IgG1, κ APC isotype control (bottom).
  • 4B10_APC_T-Bet_Antibody_ICFC_2_021715
See APC spectral data
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644813 25 µg 140,00€
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644814 100 µg 280,00€
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Description

T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).

Application References

(PubMed link indicates BioLegend citation)
  1. Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB)
  2. Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP)
  3. Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF)
  4. Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
Product Citations
  1. Lu Y, et al. 2018. Br J Pharmacol. 175:1085. PubMed
  2. Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed
  3. Romain Bouziat et al. 2018. Cell host & microbe. 24(5):677-688 . PubMed
  4. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  5. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  6. Cuadrado E, et al. 2018. Immunity. 48:1046. PubMed
  7. Cao Y, et al. 2019. Nat Commun. 10:1280. PubMed
  8. Kelsey E Sivick et al. 2018. Cell reports. 25(11):3074-3085 . PubMed
  9. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  10. Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
  11. Holly Bachus et al. 2018. Immunity. 50(1):225-240 . PubMed
  12. Raju S, et al. 2020. Cell Reports. 29(9):2556-2564.e3.. PubMed
  13. Kumar R, et al. 2020. Cell Reports. 30(8):2512-2525. PubMed
  14. Xia S, et al. 2014. J Leukoc Biol. 95:733. PubMed
  15. Domeier P, et al. 2016. J Exp Med. 213: 715 - 732. PubMed
  16. LM S, et al. 2016. Cell Rep. 16(12): 3286-96. PubMed
  17. Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
  18. Ren Z, et al. 2021. EMBO Molecular Medicine. :e14059. PubMed
  19. Si J, et al. 2020. Cancer Cell. 38(4):551-566.e11. PubMed
  20. Giampazolias E, et al. 2021. Cell. . PubMed
  21. Wagner A, et al. 2021. Cell. . PubMed
RRID
AB_10896913 (BioLegend Cat. No. 644813)
AB_10901173 (BioLegend Cat. No. 644814)

Antigen Details

Structure
T-box transcription factor, approximately 58 kD.
Distribution

Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.

Function
Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
Cell Type
B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Szabo SJ, et al. 2000. Cell 100:655.
2. Szabo SJ, et al. 2002. Science 295:338.
3. Finotto S, et al. 2002. Science 295:336.
4. Mullen AC, et al. 2001. Science 292:1907.

Gene ID
30009 View all products for this Gene ID
UniProt
View information about T-bet on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 02.17.2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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