Alexa Fluor® 647 anti-mouse CD18 Antibody

Pricing & Availability
Clone
M18/2 (See other available formats)
Other Names
integrin β2, β2 integrin, ITGB2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
M18slash2_Alx647_080207
C57BL/6 mouse splenocytes stained with M18/2 Alexa Fluor® 647
  • M18slash2_Alx647_080207
    C57BL/6 mouse splenocytes stained with M18/2 Alexa Fluor® 647
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
101414 100 µg 156€
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Description

CD18 is a 95 kD protein, also known as integrin β2 subunit. It is expressed on all leukocytes. CD18, in association with integrin α chain CD11a, CD11b, and CD11c forms LFA-1, Mac-1, and αXβ2, respectively, and plays an important role in leukocytes adhesion. The CD18 integrin complexes bind ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, and fibrinogen. The M18/2 antibody is able to block tumor cell metastasis and enhance cell adhesion.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CTL glycoprotein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC , IF - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1, in vitro and in vivo blocking of tumor cells metastasis or stimulation of cell-adhesion2-6, and immunohistochemical staining7 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 101410).

Application References

(PubMed link indicates BioLegend citation)
  1. Sanchez-Madrid, et al. 1983. J. Exp. Med. 158:586. (IP, WB)
  2. Zahalka MA, et al. 1993. J. Immunol. 150:4466. (IP, Block, Stim)
  3. Zahalka MA, et al. 1994. Int. Immunol. 6:917. (Block, Stim)
  4. Ishida Y, et al. 1994. Cell. Immunol. 155:414. (Block, Stim)
  5. Isobe M, et al. 1992. Science 255:1125. (Block, Stim)
  6. Driessens MHE, et al. 1996. J. Leukoc. Biol. 60:758. (Block, Stim)
  7. Barlow SC, et al. 2004. Amer. J. Pathol. 165:1849. (IHC)
RRID
AB_2265032 (BioLegend Cat. No. 101414)

Antigen Details

Structure
Integrin family, associates with CD11a, CD11b, CD11c, 95 kD
Distribution

All leukocytes

Function
Adhesion
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, fibrinogen
Cell Type
Leukocytes, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175.
3. Wilson RW, et al. 1993. J. Immunol. 151:1571.

Gene ID
16414 View all products for this Gene ID
UniProt
View information about CD18 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 06.27.2014

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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