- SMI 32 (See other available formats)
- Other Names
- Neurofilament heavy polypeptide, NF-H, 200 kD neurofilament protein, neurofilament triplet H protein
- Mouse IgG1, κ
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- Product Citations
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Neurofilaments (NF) are approximately 10 nanometer intermediate filaments found in neurons. They are a major component of the neuronal cytoskeleton, and function primarily to provide structural support for the axon and to regulate the axon diameter. There are three major NF subunits, and the names given to these subunits are based upon the apparent molecular mass of the mammalian subunits on SDS-PAGE. The light or lowest NF (NF-L) runs at 68-70 kD. The medium or middle NF (NF-M) runs at about 145-160 kD, and the heavy or highest NF (NF-H) runs at 200-220 kD. However, the actual molecular weight of these proteins is considerably lower due to the highly charged C-terminal regions of the molecules. The level of NF gene expression correlates with the axonal diameter, which controls how fast electrical signals travel down the axon. Mutant mice with NF abnormalities have phenotypes resembling amyotrophic lateral sclerosis. NF immunostaining is common in diagnostic neuropathology. It is useful for differentiating neurons (positive for NF) from the glia (negative for NF).Product Details
- Human, Mouse, Rat
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 594.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
IHC-P - Quality tested
ICC, IHC-F - Validated
- Recommended Usage
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry (formalin-fixed paraffin-embedded), a concentration range of 5.0 - 10.0 µg/ml is suggested. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/ml is recommended. For immunohistochemical staining on frozen tissue sections, a concentration range of 5.0 - 10.0 µg/ml is suggested.It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Application Notes
Additional reported applications (for the relevant formats) include Western blotting6, immunohistochemistry4,5, immunocytochemistry1,2,3, 7, array tomography8.
Cross-reactivity to monkey tissue has been reported in the literature4.
This antibody reacts with a nonphosphorylated epitope in neurofilament H of most mammalian species. The reaction is masked when the epitope is phosphorylated. The staining of isolated neurofilament preparations is greatly intensified upon dephosphorylation. Immunocytochemically, SMI 32 visualizes neuronal cell bodies, dendrites, and some thick axons in the central and peripheral nervous systems. However, thin axons are not revealed. Other cells and tissues are unreactive. The antibody distinguishes three subdivisions of the macaque precentral motor cortex. The greater size of the left versus the right superior temporal lobe was found to be due to increased axonal myelination and not due to increased number of glial cells or SMI 32-enumerated neurons, suggesting that the specialization for language in the left temporal lobe is related to increased speed of signal transmission. In cultures of murine cortex, SMI 32 labels a neuronal population with enhanced vulnerability to kainate toxicity most of which are GABAergic and reveal kainate-activated Ca2+ uptake.
(PubMed link indicates BioLegend citation)
- Chang Q, Martin LJ. 2011. J. Neurosci., 31:2815-27. (ICC) PubMed
- Stevens HE, et al. 2010. J. Neurosci. 30:5590-602. (ICC) PubMed
- Kiryu-Seo S, et al. 2010. J. Neurosci. 30:6658-66. (ICC) PubMed
- Redondo J, et al. 2015. Brain Pathol. 25(6):692. (IHC-P) PubMed
- Feng L, et al. 2017. eNeuro. 4(1): 0331-16.2016. (IHC-P) PubMed
- Feng L, et al. 2014. Invest Ophthalmol Vis Sci. 54(2): 1106:1117. (IHC-P) PubMed
- Theotokis, et al. 2016. J. Neuroinflammation 13(1):265 (IHC-P)
- Bennett, et al. 2015. J. Neurosci. Methods 245:25-36 (Array Tomography)
- Petzold A, et al. 2011. Brain 134:464. (WB) PubMed
AB_2721392 (BioLegend Cat. No. 801709)
AB_2721393 (BioLegend Cat. No. 801710)
- Neurofilament H has an apparent molecular mass of 200-220 kD.
Tissue distribution: CNS, peripheral nerves and glandular cells of the prostate
Cellular distribution: Cytoskeleton, nucleus, cytosol, and mitochondrion
- NF-H Neurofilaments are the major components of the neuronal cytoskeleton. They provide axonal support and regulate axon diameter. Phosphorylation of NF-H results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
- Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation result in changes to the neurofilament function.
- Cell Type
- Mature Neurons
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Intermediate Filaments, Phospho-Proteins
- Antigen References
- Turner M, et al. 2015. Journal of Neuroimmunology. 285: 4. PubMed
- Pagliarini V, et al. 2015. J. Cell Biol.. 211: 77. PubMed
- Petzold A, et al. 2011. Brain 134. (WB) PubMed
- Yuan A, et al. 2016. Brain Res Bull 126(3): 334.
- Parlakian A, et al. 2016. Rev Neurol. 172(10): 607.
- Li D, et al. 2016. Front Aging Neurosci. 8: 290.
- Costa J, et al. 2016. Clin Chim Acta. 455: 7.
- Lad SP, et al. 2010. J Stroke Cerebrovasc Dis. 21(1): 30.
- Gene ID
- 4744 View all products for this Gene ID
- View information about Neurofilament H (NF-H) on UniProt.org
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