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Purified anti-Neurofilament H (NF-H), Nonphosphorylated Antibody (Previously Covance catalog# SMI-32P)

Pricing & Availability
Clone
SMI 32 (See other available formats)
Regulatory Status
RUO
Other Names
Neurofilament heavy polypeptide, NF-H, 200 kD neurofilament protein, neurofilament triplet H protein
Previously
Covance Catalog# SMI-32P
Isotype
Mouse IgG1
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Product Citations
publications
1_SMI-32_PURE_NF-H_Antibody_IHC-P_011218
IHC staining of purified anti-Neurofilament H (NF-H), Nonphosphorylated antibody (clone SMI 32) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieval-ALL Antigen Unmasking System 3 (Cat. No. 927601), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50µm
  • 1_SMI-32_PURE_NF-H_Antibody_IHC-P_011218
    IHC staining of purified anti-Neurofilament H (NF-H), Nonphosphorylated antibody (clone SMI 32) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieval-ALL Antigen Unmasking System 3 (Cat. No. 927601), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50µm
  • SMI-32_PURE_NeurofilamentH_Antibody_HR_2_090517
    Immunofluorescence staining of anti-Neurofilament H (NF-H), Nonphosphorylated antibody (clone SMI 32) on formalin-fixed paraffin-embedded (FFPE) tissue section from mouse Thalamus. After antigen retrieval using Retrieval-ALL Antigen Unmasking System 3 (Cat. No. 927601), the tissue was blocked with Normal Serum Block for 30 min at room temperature, and then incubated with SMI 32 at 5 µg/mL overnight at 4°C, followed by incubation with Alexa Fluor® 488 Goat anti-mouse IgG (Cat. No. 405319) for one hour at room temperature. The image was captured with a 40X objective (Scale Bar: 20 µm).
  • SMI-32_PURE_NeurofilamentH_Antibody_HR_3_090517
    IHC-Fluorescence staining of Formalin Fixed Paraffin Embedded (FFPE) Rat Cerebellum by anti-Neurofilament H (NF-H), Nonphosphorylated Antibody clone SMI 32. After antigen retrieval using Retrieval-ALL Antigen Unmasking System 3 (Cat. No. 927601), the tissue was blocked with Normal Serum Block for 30 min at room temperature, and then incubated with SMI 32 at 5 µg/mL overnight at 4°C, followed by incubation with Alexa Fluor® 488 Goat anti-mouse IgG (Cat. No. 405319) for one hour at room temperature. The image was captured with a 40X objective (Scale Bar: 20 µm).
  • SMI-32_PURE_NF-H_Antibody_WB_011218
    Western blot of purified anti-Neurofilament H (NF-H), Nonphosphorylated antibody (clone SMI 32). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 ug/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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801702 25 µL 90€
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Description

Neurofilaments (NF) are approximately 10 nanometer intermediate filaments found in neurons. They are a major component of the neuronal cytoskeleton, and function primarily to provide structural support for the axon and to regulate the axon diameter. There are three major NF subunits, and the names given to these subunits are based upon the apparent molecular mass of the mammalian subunits on SDS-PAGE. The light or lowest NF (NF-L) runs at 68-70 kD. The medium or middle NF (NF-M) runs at about 145-160 kD, and the heavy or highest NF (NF-H) runs at 200-220 kD. However, the actual molecular weight of these proteins is considerably lower due to the highly charged C-terminal regions of the molecules. The level of NF gene expression correlates with the axonal diameter, which controls how fast electrical signals travel down the axon. Mutant mice with NF abnormalities have phenotypes resembling amyotrophic lateral sclerosis. NF immunostaining is common in diagnostic neuropathology. It is useful for differentiating neurons (positive for NF) from the glia (negative for NF).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
WB - Verified
Array Tomography, ICC - Reported in the literature, not verified in house
SB - Community verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include Western blotting6, immunohistochemistry4,5, immunocytochemistry1,2,3, 7, array tomography8.

Cross-reactivity to monkey tissue has been Reported in the literature, not verified in house4.

This antibody reacts with a nonphosphorylated epitope in neurofilament H of most mammalian species. The reaction is masked when the epitope is phosphorylated. The staining of isolated neurofilament preparations is greatly intensified upon dephosphorylation. Immunocytochemically, SMI 32 visualizes neuronal cell bodies, dendrites, and some thick axons in the central and peripheral nervous systems. However, thin axons are not revealed. Other cells and tissues are unreactive. The antibody distinguishes three subdivisions of the macaque precentral motor cortex. The greater size of the left versus the right superior temporal lobe was found to be due to increased axonal myelination and not due to increased number of glial cells or SMI 32-enumerated neurons, suggesting that the specialization for language in the left temporal lobe is related to increased speed of signal transmission. In cultures of murine cortex, SMI 32 labels a neuronal population with enhanced vulnerability to kainate toxicity most of which are GABAergic and reveal kainate-activated Ca2+ uptake.

Additional Product Notes

This product has been verified for IHC-P (Immunohistochemistry - formalin-fixed paraffin-embedded tissues) on the NanoString GeoMx® Digital Spatial Profiler. The GeoMx® enables researchers to perform spatial analysis of protein and RNA targets in FFPE and fresh frozen human and mouse samples. For more information about our spatial biology products and the GeoMx® platform, please visit our spatial biology page.

Application References

(PubMed link indicates BioLegend citation)
  1. Chang Q, Martin LJ. 2011. J. Neurosci., 31:2815-27. (ICC) PubMed
  2. Stevens HE, et al. 2010. J. Neurosci. 30:5590-602. (ICC) PubMed
  3. Kiryu-Seo S, et al. 2010. J. Neurosci. 30:6658-66. (ICC) PubMed  
  4. Redondo J, et al. 2015. Brain Pathol. 25(6):692. (IHC-P) PubMed  
  5. Feng L, et al. 2017. eNeuro. 4(1): 0331-16.2016. (IHC-P) PubMed
  6. Feng L, et al. 2014. Invest Ophthalmol Vis Sci. 54(2): 1106–1117. (IHC-P) PubMed
  7. Theotokis, et al. 2016. J. Neuroinflammation 13(1):265 (IHC-P)
  8. Bennett, et al. 2015. J. Neurosci. Methods 245:25-36 (Array Tomography)
  9. Petzold A, et al. 2011. Brain 134:464. (WB) PubMed  
Product Citations
  1. Cignarella F et al. 2018. Cell metabolism. 27(6):1222-1235 . PubMed
  2. Zhou Y, et al. 2019. J Clin Invest. 130:1756. PubMed
  3. Abernathy DG et al. 2017. Cell stem cell. 21(3):332-348 . PubMed
  4. Zhang LY, et al. 2020. Theranostics. 0.468055556. PubMed
  5. Castelli LM, et al. 2021. Mol Neurodegener. 16:53. PubMed
  6. Tsolias A, et al. 2022. Front Neural Circuits. 15:795325. PubMed
  7. Munot P, et al. 2021. Neuropathol Appl Neurobiol. Online ahead of print. PubMed
  8. Stevens H, et al. 2010. J Neurosci. 30:5590-5602. PubMed
  9. Cocozza G, et al. 2018. Brain Behav Immun. 73:584. PubMed
  10. Penndorf D et al. 2017. PloS one. 12(8):e0183684 . PubMed
  11. Ruff T, et al. 2021. Front Mol Neurosci. 14:790466. PubMed
  12. Bakken TE, et al. 2021. Nature. 598:111. PubMed
  13. Rosenkranz SC, et al. 2021. eLife. 10:00. PubMed
  14. Atkinson R, et al. 2021. Dis Model Mech. 14:dmm047548. PubMed
  15. Sherafat A, et al. 2021. Nat Commun. 12:2265. PubMed
  16. Manivasagam S, et al. 2022. J Immunol. 208:1341. PubMed
  17. Leo M, et al. 2022. Cells. 11:. PubMed
  18. Li X, et al. 2017. Mol Ther. 25(1):140-152. PubMed
  19. Trikamji B, et al. 2021. Muscle Nerve. 63:506. PubMed
  20. Ding B, et al. 2021. J Neurosci. 41:2024. PubMed
  21. Mahajan KR, et al. 2020. Ann Neurol. 88:81. PubMed
  22. Mikhalkin A, et al. 2020. Journal of Comparative Neurology. 529(7):1430-1441. PubMed
  23. Yoo M, Kim T 2016. Sci Rep. 6:28548. PubMed
  24. Corsini S, et al. 2017. Cell Death Dis. 10.1038/cddis.2017.232. PubMed
  25. Wong R, et al. 2019. Brain Behav Immun. 76:126. PubMed
  26. Zhang Q, et al. 2019. PLoS Biol. 17:e3000330. PubMed
  27. Drohomyrecky PC, et al. 2019. J Immunol. 203:2588. PubMed
  28. Gaja-Capdevila N, et al. 2021. Front Pharmacol. 12:780588. PubMed
  29. Sainio MT, et al. 2022. Front Cell Dev Biol. 9:820105. PubMed
  30. Shi L, et al. 2021. Immunity. . PubMed
  31. Iida M, et al. 2019. Nat Commun. 10:4262. PubMed
  32. Doust YV, et al. 2021. Front Neurol. 12:722526. PubMed
  33. Utagawa EC, et al. 2022. Acta Neuropathol Commun. 10:86. PubMed
  34. Shi X, et al. 2021. Nat Commun. 12:6943. PubMed
  35. Prukop T, et al. 2020. J Neurosci Res. 1933:98. PubMed
  36. Brodie-Kommit J, et al. 2021. Science Advances. 7(11):. PubMed
  37. Saba L, et al. 2016. Cereb Cortex. 26: 1512-1528. PubMed
  38. Li S, et al. 2016. Proc Natl Acad Sci U S A. 113: 1937 - 1942. PubMed
  39. Reinhard K, et al. 2020. eLife. 8:e50697.. PubMed
  40. Honig MG, et al. 2021. Front Neurosci. 15:701317. PubMed
  41. , et al. 2021. Nature. 598:151. PubMed
  42. Kondo T, et al. 2022. Front Cell Neurosci. 16:858562. PubMed
  43. Harley J, et al. 2021. Brain Commun. 3:fcab166. PubMed
  44. Calvo-Barreiro L, et al. 2021. Neurotherapeutics. . PubMed
  45. Merkulyeva N, et al. 2021. Neuroscience Letters. 762:136165. PubMed
  46. Laug D, et al. 2019. J Clin Invest. 129:4408. PubMed
  47. Takakura K, et al. 2017. Heliyon. 3:e00462. PubMed
  48. Tung YT et al. 2019. Cell Stem Cell. 25(2):193-209 . PubMed
  49. Ondatje BN, et al. 2022. Lab Chip. 22:4246. PubMed
  50. Sharf T, et al. 2022. Nat Commun. 13:4403. PubMed
  51. Mavlyutov TA, et al. 2022. Cell Biosci. 12:72. PubMed
  52. Chen D, et al. 2022. J Neuroinflammation. 19:112. PubMed
  53. Clark CM, et al. 2021. Brain Sci. 11:. PubMed
  54. Simone R, et al. 2021. Nature. 594:117. PubMed
  55. Candadai AA, et al. 2021. PLoS One. e0247901:16. PubMed
  56. Ho R, et al. 2020. Cell Systems. 12(2):159-175.e9. PubMed
  57. Rizzo F, et al. 2016. Hum Mol Genet. 10.1093/hmg/ddw258. PubMed
  58. örner S, et al. 2016. J Neuropathol Exp Neurol. 10.1093/jnen/nlw003. PubMed
  59. Yi‐Lan Weng et al. 2018. Neuron. 97(2):313-325 . PubMed
  60. McLeod VM et al. 2019. Br J Pharmacol. 176(13):2111-2130 . PubMed
  61. Schirmer L, et al. 2019. Nature. 573:75. PubMed
  62. Yang C, et al. 2020. Neuron. 105:276. PubMed
  63. Abo-Rady M, et al. 2020. Stem Cell Reports. 14:390. PubMed
  64. Risner ML, et al. 2022. Mol Neurobiol. 59:1366. PubMed
  65. Yu M, et al. 2022. Front Neurol. 13:903565. PubMed
  66. Ou Y, et al. 2016. J Neurosci. 36: 9240 - 9252. PubMed
  67. Himmelein S, et al. 2017. J Virol. 10.1128/JVI.00331-17. PubMed
  68. Miguel JC, et al. 2021. Front Aging Neurosci. 645334:13. PubMed
  69. Saraf MP et al. 2019. The Journal of comparative neurology. 527(15):2599-2611 . PubMed
  70. Ben Haim L, et al. 2021. Glia. 69:2812. PubMed
  71. van der Heijden ME, et al. 2021. J Physiol. 599:2037. PubMed
  72. Jiang D, et al. 2019. Journal of Comparative Neurology. 528(5):729-755. PubMed
  73. Azeez I, et al. 2016. J Neuropathol Exp Neurol. 75: 843 - 854. PubMed
  74. Donkels C, et al. 2016. Cereb Cortex. 10.1093/cercor/bhv346. PubMed
  75. Kinoshita H, et al. 2019. Sci Rep. 9:11519. PubMed
  76. Groh J, et al. 2021. Brain Commun. 3:fcab047. PubMed
  77. Petrozziello T, et al. 2017. Cell Death Differ. 10.1038/cdd.2016.154. PubMed
  78. Chavali M, et al. 2020. Neuron. . PubMed
  79. Roboon J, et al. 2019. Front Cell Neurosci. 13:258. PubMed
  80. Okigawa S, et al. 2021. J Comp Neurol. 529:2099. PubMed
  81. Lanz T, et al. 2017. Sci Rep. 7:41271. PubMed
  82. Brambilla L, et al. 2016. Hum Mol Genet. 10.1093/hmg/ddw161. PubMed
  83. Ebert T 2016. Hum Mol Genet. 25: 514 - 523. PubMed
  84. Ito K, et al. 2018. Sci Rep. 33:1052. PubMed
  85. Saraf MP et al. 2018. The Journal of comparative neurology. 527(3):625-639 . PubMed
  86. Hirono M, et al. 2018. J Neurosci. 38:6130. PubMed
  87. Reinehr S, et al. 2019. Int J Mol Sci. 2.613194444. PubMed
  88. Tan H, et al. 2019. Cell Death Differ. 27:1369. PubMed
  89. van der Heijden ME, et al. 2021. Elife. 10:. PubMed
  90. Wolf C, et al. 2022. Commun Biol. 5:541. PubMed
  91. Parisi C, et al. 2016. Cell Death Differ. 23:531-541. PubMed
  92. Wagener R, et al. 2016. Cereb Cortex. 26: 820 - 837. PubMed
  93. Ho R, et al. 2016. Nat Neurosci. 10.1038/nn.4345. PubMed
  94. Hughes RO, et al. 2021. Cell Reports. 34(1):108588. PubMed
  95. Korzhevskii DE, et al. 2017. Zh Nevrol Psikhiatr Im S S Korsakova. 117:50. PubMed
  96. Dyer M, et al. 2019. Front Aging Neurosci. 11:68. PubMed
  97. Larson VA et al. 2018. eLife. 7 pii: e34829. PubMed
  98. Kelley KW et al. 2018. Neuron. 98(2):306-319 . PubMed
  99. Puller C, et al. 2020. J Neurosci. 40:1302. PubMed
  100. Casanovas A, et al. 2017. Sci Rep. 7:40155. PubMed
  101. Berry R, et al. 2015. PLoS One. 10: 0144341. PubMed
  102. Wang H, et al. 2015. Sci Rep. 5: 17383. PubMed
  103. De Pace R, et al. 2018. PLoS Genet. 8:6458. PubMed
  104. Jiang LL, et al. 2019. J Clin Invest. 130. PubMed
  105. Nelke A, et al. 2022. Front Pharmacol. 12:773925. PubMed
  106. Thiry L, et al. 2022. ASN Neuro. 14:17590914211073381. PubMed
  107. Whye D, et al. 2022. Curr Protoc. 2:e568. PubMed
  108. Martin Q 2011. J Neurosci. 31:2815-2827. PubMed
  109. Petzold A, et al. 2011. Brain. 134:464-483. PubMed
  110. Redondo J, et al. 2015. Brain Pathol. 25:692-700. PubMed
  111. Faustino Martins JM, et al. 2020. Cell Stem Cell. 172:26. PubMed
  112. Miao W, et al. 2020. J Immunol. 1486:204. PubMed
  113. Fouda AY, et al. 2020. Invest Ophthalmol Vis Sci. 51:61. PubMed
  114. Cuadrado E et al. 2019. Cell reports. 26(7):1718-1726 . PubMed
  115. Maruyama T, et al. 2018. Cell Death Dis. 12:146. PubMed
  116. Pelisch N, et al. 2021. Eneuro. 8:. PubMed
  117. Swanson OK, et al. 2021. Eneuro. 8:. PubMed
  118. Alpár A, et al. 2018. EMBO J. 37:. PubMed
  119. Alcover-Sanchez B, et al. 2021. Glia. 69:619. PubMed
  120. Sen T, et al. 2020. J Neurosci. 40:424. PubMed
  121. Litvina EY et al. 2017. Neuron. 96(2):330-338 . PubMed
  122. Wang F, et al. 2021. Current Biology. 31(11):2263-2273.e3. PubMed
  123. Bukreeva I, et al. 2017. Sci Rep. 7:41054. PubMed
  124. Feng L, et al. 2017. eNeuro. 4(1). PubMed
  125. MacNair L, et al. 2016. Brain. 139: 86 - 100. PubMed
  126. Wood KC et al. 2017. PloS one. 12(1):e0170264 . PubMed
  127. Abbas Farishta R, et al. 2021. Cereb Cortex Commun. 1:tgaa030. PubMed
  128. Turner M, et al. 2015. J Neuroimmunol. 285: 4-12. PubMed
  129. Pagliarini V, et al. 2015. J Cell Biol. 211: 77 - 90. PubMed
  130. Kiryu-Seo S, et al. 2010. J Neurosci. 30:6658-6666. PubMed
  131. Luna G, et al. 2016. Exp Eye Res. 150: 4-21. PubMed
  132. Roboon J, et al. 2021. Journal of Neurochemistry. . PubMed
  133. Eixarch H, et al. 2020. Neurotherapeutics. 17:1988. PubMed
  134. Griggs WS et al. 2017. Frontiers in neuroanatomy. 0.531944444 . PubMed
  135. Krieger B, et al. 2017. PLoS One. 12:e0180091. PubMed
  136. Granatiero V, et al. 2021. Autophagy. 17:4029. PubMed
  137. Ryan BJ, et al. 2021. J Neurosci. 41:3731. PubMed
  138. Khandker L, et al. 2022. Cell Rep. 38:110423. PubMed
  139. Niu F, et al. 2022. Elife. 11:. PubMed
  140. Henschke JU, et al. 2021. Cell Calcium. 96:102390. PubMed
  141. Richard P, et al. 2020. Autophagy. :1. PubMed
  142. Doroshenko ER, et al. 2021. Front Immunol. 570425:12. PubMed
  143. Wegscheid ML, et al. 2021. Cell Reports. 36(1):109315. PubMed
  144. Salapa HE, et al. 2019. Journal of Neuroscience Research. 98:704. PubMed
  145. Sepehrimanesh M, et al. 2020. American Journal of Physiology-Cell Physiology. 319(4):C771-C780. PubMed
  146. Lv Q, et al. 2021. Cereb Cortex. 31:341. PubMed
RRID
AB_2715852 (BioLegend Cat. No. 801702)
AB_2564642 (BioLegend Cat. No. 801701)

Antigen Details

Structure
Neurofilament H has an apparent molecular mass of 200-220 kD.
Distribution

Tissue distribution: CNS, peripheral nerves and glandular cells of the prostate
Cellular distribution: Cytoskeleton, nucleus, cytosol, and mitochondrion

Function
NF-H Neurofilaments are the major components of the neuronal cytoskeleton. They provide axonal support and regulate axon diameter. Phosphorylation of NF-H results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Receptors
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation result in changes to the neurofilament function.
Cell Type
Mature Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments, Phospho-Proteins
Antigen References
  1. Turner M, et al. 2015. Journal of Neuroimmunology. 285: 4. PubMed
  2. Pagliarini V, et al. 2015. J. Cell Biol.. 211: 77. PubMed
  3. Petzold A, et al. 2011. Brain 134. (WB) PubMed 
  4. Yuan A, et al. 2016. Brain Res Bull  126(3): 334.
  5. Parlakian A, et al. 2016. Rev Neurol. 172(10): 607.
  6. Li D, et al. 2016. Front Aging Neurosci. 8: 290.
  7. Costa J, et al. 2016. Clin Chim Acta. 455: 7.
  8. Lad SP, et al. 2010.  J Stroke Cerebrovasc Dis. 21(1): 30.
Gene ID
4744 View all products for this Gene ID
UniProt
View information about Neurofilament H on UniProt.org

Related FAQs

If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?

It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.

Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?

Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.

Are other fluorophores compatible with IBEX?

Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.

The same antibody works in one tissue type but not another. What is happening?

Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.

How can I be sure the staining I’m seeing in my tissue is real?

In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.

Go To Top Version: 3    Revision Date: 01.23.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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