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Purified anti-PIK3R2 Antibody

Pricing & Availability
Clone
W17020A (See other available formats)
Regulatory Status
RUO
Other Names
Phosphoinositide-3-kinase regulatory subunit 2 P85B
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W17020A_Purified_PIK3R2_Antibody_021319.png
Total cell lysates (15 µg protein) from the indicated cell lines were resolved using 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with purified anti-PIK3R2 antibody (clone W17020A) at 1.0 µg/mL (1:500 dilution). Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat-IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Equal protein loading was confirmed using Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607903) at a 1:50,000 dilution (lower). Lane M: MW ladder. Cell lysates were loaded in order of increasing predicted PIK3R2 mRNA expression levels (source: Human Protein Atlas)
  • W17020A_Purified_PIK3R2_Antibody_021319.png
    Total cell lysates (15 µg protein) from the indicated cell lines were resolved using 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with purified anti-PIK3R2 antibody (clone W17020A) at 1.0 µg/mL (1:500 dilution). Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat-IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Equal protein loading was confirmed using Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607903) at a 1:50,000 dilution (lower). Lane M: MW ladder. Cell lysates were loaded in order of increasing predicted PIK3R2 mRNA expression levels (source: Human Protein Atlas)
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667001 25 µg £70
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667002 100 µg £174
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Description

Phosphoinositide-3-kinase (PI3 kinase) is a lipid kinase that plays a critical role in cell signaling through generation of phosphoinositides at the plasma membrane in response to growth factor and hormone stimulation. Activation of PI3K is essential for cell growth, migration, motility, and survival. PI3K is comprised of a catalytic subunit, p110, that harbors enzymatic activity and a regulatory subunit, p85, that promotes both p110 stability and inhibition of p85. Moreover, p85 recognizes phosphorylated growth factor receptors via 2 SH2 domains that bring the PI3K dimer to the plasma membrane and in closer proximity to its substrate. Two p85 isoforms, p85α and p85β, have been described. Elevated expression of PIK3R2 (p85β) has been described in breast and colon cancers and is associated with increased tumorigenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human PIK3R2 (aa 426-621)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone failed to immunoprecipitate PIK3R2 from MCF-7 lysates. We therefore do not recommend W17020A for this application.

This clone failed to stain PIK3R2 from PFA-fixed and methanol permeabilized MCF-7 cells when tested by ICC. We therefore do not recommend W17020A for this application.

Due to pockets of high sequence similarity between the immunizing sequence and PIK3R1, this clone may cross-react with PIK3R1.

RRID
AB_2801126 (BioLegend Cat. No. 667001)
AB_2801127 (BioLegend Cat. No. 667002)

Antigen Details

Structure
PIK3R2 is a 728 amino acid protein with a predicted molecular weight of 81.5 kD
Distribution

Ubiquitously expressed/cytosolic localization

Function
Cell Signaling
Interaction
p110, FBXL2, PTPN13, XBP1
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Otsu M, et al. 1991. Cell. 65:91.
  2. Geering B, et al. 2007. Proc Natl Acad Sci. 104:7809.
  3. Cortés I, et al. 2012. Proc Natl Acad Sci. 109:11318.
  4. Ito Y, et al. 2014. Proc Natl Acad Sci. 111:16826.
Gene ID
5296 View all products for this Gene ID
UniProt
View information about PIK3R2 on UniProt.org
Go To Top Version: 1    Revision Date: 02/13/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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